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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

The LC50 at 96 h was calculated as 21.17 mg/L (Guidelines for Hazard Evaluation of New Chemical Substances, Environmental Protection Agency of P.R.C. (HJ/T 154 -2004)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
21.17 mg/L

Additional information

METHOD

An acute toxicity study was conducted in zebra fish (Brachydanio rerio) under semi-static conditions and, based on results from preliminary testing, concentration levels of the test item were selected as 9.70, 14.85, 22.75, 34.83 and 53.35 mg/L (factor of 1.53). A control group was used in parallel and three replicates for each group (including the test groups and the control group) were performed. The water was filtrated with an active carbon filter (PCF-1200, made in Japan). Concentrations of the test item, dissolved oxygen and pH were determined at the beginning of the test and at 24 hour intervals during the 96 hour exposure period. Water hardness and total alkalinity were measured at the beginning of the test in the control. During the 96 hour exposure period, toxic signs and mortality were observed and recorded.

RESULTS

The LC50 at 96 h was calculated as 21.17 mg/L and the 95 % confidence limits were 17.50 - 26.63 mg/L based on mortality. Based on this result and the fish toxicity grading criteria in the Guidelines for Hazard Evaluation of New Chemical Substances, Environmental Protection Agency of P.R.C. (HJ/T 154 -2004), the test material is graded as a chemical with middle fish toxicity under the experimental condition of the study.