Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report Date:
1995

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: EEC Directive 79/831/EEC, Annex V, Part C
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Asta-C15-Acetal (C15-Acl)
- Lot/batch No.: 25656/133 S
- Analytical purity: about 95 %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution (100 mg/l) was prepared by stirring the test substance in the test medium for about 20 hours at 20 °C.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2-24 h

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.20 - 3.20 mmol/l
Test temperature:
18.9 - 21.8 °C
pH:
7.9 - 8.1
Dissolved oxygen:
8.4 - 9.3 mg/l
Nominal and measured concentrations:
Nominal test substance concentrations:
6.25, 12.5, 25, 50 and 100 mg/l

Analytically determined test concentrations:
exposure time 0-24 h
after 0 h: 5.8 mg/l (nominal 6.25 mg/l), 24.2 mg/l (nominal 25 mg/l), 101.3 mg/l (nominal 100 mg/l)
after 24 h (stocked with animals): 5.6 mg/l (nominal 6.25 mg/l), 23.3 mg/l (nominal 25 mg/l), 95.9 mg/l (nominal 100 mg/l)
Exposure time 24-48 h
after 0 h: 5.8 mg/l (nominal 6.25 mg/l), 23.9 mg/l (nominal 25 mg/l), 96.0 mg/l (nominal 100 mg/l)
after 24 h (stocked with animals): 5.4 mg/l (nominal 6.25 mg/l), 23.5 mg/l (nominal 25 mg/l), 95.7 mg/l (nominal 100 mg/l)
Details on test conditions:
TEST SYSTEM
- Test vessel: flat bottom glass tubes
- Fill volume: 10 ml
- Renewal rate of test solution (frequency/flow rate): after 24 hours
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic M4 medium
- Alkalinity: 0.8 - 1.0 mmol/l
- Ca/mg ratio: 4:1
- Conductivity: 550 - 650 µS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: About 5 - 6 µE/(m²s) in the range of 400 - 700 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 3, 6, 24 and 48 hours

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The test substance is not stable in the test medium.

Applicant's summary and conclusion