(E)-3-formylbut-2-enyl acetate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study, comparable to OECD 202. Scientifically acceptable.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

Cited as Directive 84/449/EEC, C.2 (Acute toxicity for Daphnia)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

(E)-3-formylbut-2-enyl acetate

EC Number:

247-825-4

EC Name:

(E)-3-formylbut-2-enyl acetate

Cas Number:

26586-02-7

Molecular formula:

C7H10O3

IUPAC Name:

(2E)-3-methyl-4-oxobut-2-en-1-yl acetate

Details on test material:

- Name of test material (as cited in study report): C5-acetat

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test medium of the highest test concentration (nominal 100 mg/l) was prepared. Then, a serial dilution of this test concentration in culture medium was prepared to obtain the desired test concentrations. In addition, a control was tested in parallel.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Daphnia magna Straus, originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978
- Age at study initiation (mean and range, SD): 2 - 24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

19 - 21 °C

pH:

7.8 - 8.2

Dissolved oxygen:

7.8 - 9.2 mg/l

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
1.56, 3.12, 6.24, 12.5, 25, 49.9, 100, control

Details on test conditions:

TEST SYSTEM
- Test vessel: flat bottom glass tubes
- Type (delete if not applicable): open / closed
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to the test guideline 79/831 EWG, Attachment V, C.2


OTHER TEST CONDITIONS
- Photoperiod: 16:8 hours
- Light intensity: diffuse light (10 microeinstein/m*m*s) at 400 - 750 nm wave length


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 3, 6, 24 and 48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

6.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

11.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

49.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

Probit analysis.

Any other information on results incl. tables

No. of immobile daphnids after x hours in %:
Nominal test concentration (mg/l) / 0 / 3/ 6 / 24 / 48 /
Control /   0 /   0 /   0 /   0 /   0 /
Solvent /   0 /   0 /   0 /   0 /   0 /
1.56    /   0 /   0 /   0 /   0 /   0 /
3.12    /   0 /   0 /   0 /   0 /   0 /
6.24    /   0 /   0 /   0 /   0 /   0 /
12.5    /   0 /   0 /   0 /  15 /  75 /
25.0    /   0 /   0 /   0 /  50 /  90 /
49.9    /   0 /   0 /   0 / 100 / 100 / 
100.0   /   0 /   5 / 100 / 100 / 100 /


24 hour ECx values:
EC0: 22.66 mg/l
EC50: 6.24 mg/l
EC100: 49.9 mg/l