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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study, comparable to OECD 202. Scientifically acceptable.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report date:
1989

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
Cited as Directive 84/449/EEC, C.2 (Acute toxicity for Daphnia)
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
(E)-3-formylbut-2-enyl acetate
EC Number:
247-825-4
EC Name:
(E)-3-formylbut-2-enyl acetate
Cas Number:
26586-02-7
Molecular formula:
C7H10O3
IUPAC Name:
(2E)-3-methyl-4-oxobut-2-en-1-yl acetate
Details on test material:
- Name of test material (as cited in study report): C5-acetat

Sampling and analysis

Analytical monitoring:
no

Test solutions

Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test medium of the highest test concentration (nominal 100 mg/l) was prepared. Then, a serial dilution of this test concentration in culture medium was prepared to obtain the desired test concentrations. In addition, a control was tested in parallel.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: Daphnia magna Straus, originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978
- Age at study initiation (mean and range, SD): 2 - 24 h

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
19 - 21 °C
pH:
7.8 - 8.2
Dissolved oxygen:
7.8 - 9.2 mg/l
Nominal and measured concentrations:
Nominal test concentrations (mg/l):
1.56, 3.12, 6.24, 12.5, 25, 49.9, 100, control
Details on test conditions:
TEST SYSTEM
- Test vessel: flat bottom glass tubes
- Type (delete if not applicable): open / closed
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to the test guideline 79/831 EWG, Attachment V, C.2


OTHER TEST CONDITIONS
- Photoperiod: 16:8 hours
- Light intensity: diffuse light (10 microeinstein/m*m*s) at 400 - 750 nm wave length


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 3, 6, 24 and 48 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
6.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
11.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
49.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Reported statistics and error estimates:
Probit analysis.

Any other information on results incl. tables

No. of immobile daphnids after x hours in %:
Nominal test concentration (mg/l) / 0 / 3/ 6 / 24 / 48 /
Control / 0 / 0 / 0 / 0 / 0 /
Solvent / 0 / 0 / 0 / 0 / 0 /
1.56 / 0 / 0 / 0 / 0 / 0 /
3.12 / 0 / 0 / 0 / 0 / 0 /
6.24 / 0 / 0 / 0 / 0 / 0 /
12.5 / 0 / 0 / 0 / 15 / 75 /
25.0 / 0 / 0 / 0 / 50 / 90 /
49.9 / 0 / 0 / 0 / 100 / 100 /
100.0 / 0 / 5 / 100 / 100 / 100 /


24 hour ECx values:
EC0: 22.66 mg/l
EC50: 6.24 mg/l
EC100: 49.9 mg/l

Applicant's summary and conclusion