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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Description of key information

 The EC50 was predicted to be 492.449523926mg/L for C.I. Basic Blue 99 in Pseudokirchnerella subcapitata for 72h by using QSAR Toolbox version 3.3

Key value for chemical safety assessment

EC50 for freshwater algae:
492.45 mg/L

Additional information

Overall reported acute toxicity studies of chemical substance and applying weight of evidence , indicate that the substance is not likely to exhibit aquatic toxicity can be classified as 'non-hazardous' as per the CLP classification criteria. 

The short term toxicity to aquatic algae for C.I. Basic Blue 99 was predicted by using QSAR Toolbox version 3.3. The EC50 was predicted to be 492.449523926mg/L for C.I. Basic Blue 99 in Pseudokirchnerella subcapitata for 72h by using QSAR Toolbox version 3.3

Based on the QSAR prediction done using the EPI Suite ECOSAR version 1.10, the 72 hours EC50 was estimated to be 15508.989 mg/L on algae for substance 3-[(4-amino-6-bromo-5,8-dihydro-1-hydroxy-8-imino-5-oxo-2-naphtyl)amino]-N,N,N-trimethylanilinium chloride (CAS no. 68123 -13 -7) with growth inhibition effects.

On the basis of available information for the target and applying weight of evidence approach, the test substance 3-[(4-amino-6 -bromo- 5,8-dihydro-1- hydroxy-8-imino-5-oxo-2-naphtyl)amino]-N,N,N-trimethylanilinium chloride cannot be considered as acute oral toxicant, in accordance with the CLP criteria. Based on the above mentioned values for the chemical, it is considered that 3-[(4-amino-6 -bromo- 5,8-dihydro-1- hydroxy-8-imino-5-oxo-2-naphtyl)amino]-N,N,N-trimethylanilinium chloride is not hazardous to the aquatic organisms at environmentally relevant concentrations and can be classified in “ not classified “as per the CLP classification.