(diethylamino)acetonitrile

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Remarks:

prior to GLP and OECD TG

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1973

Report date:

1973

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

BASF test (internal method): Usually two White Vienna rabbits were treated for 1, 5 and 15 minutes and 2 other animals for 20 hours using occlusive conditions. An application site of 2.5 X 2.5 cm was covered with the liquid or powdered and moistened test substance. In addition, skin tissue from the ear was tested by wrapping the ear. These results from the ear, however, would not be taken into account for evaluation as they do not represent testing of the dorsal/lateral flank of the back. After the application time, the skin was washed with water which sometimes contained a mild detergent. The animals were observed 7 to 8 days and skin changes were recorded on working days. The report usually describes findings after 24 hours and at the end of the observation period. Thus, for final evaluation, the findings after 48 and 72 hours from the raw data have to be taken into account.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

(diethylamino)acetonitrile

EC Number:

221-130-6

EC Name:

(diethylamino)acetonitrile

Cas Number:

3010-02-4

Molecular formula:

C6H12N2

IUPAC Name:

2-(diethylamino)acetonitrile

Details on test material:

Name of the test substance used in the study report: Produkt 73/127 dest. (= Diaethylaminoacetonitril dest.)

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

- Weight of male animals: 3.21, 3.27, and 3.12 kg
- Weight of female animal: 2.96 kg

Test system

Controls:

no

Amount / concentration applied:

Undiluted

Duration of treatment / exposure:

1, 5, and 15 minutes and 20 hours

Observation period:

8 days

Number of animals:

2 for 1, 5, and 15 minutes exposure
2 for 20 hours exposure

Details on study design:

TEST SITE
- Area of exposure: The back of the animals

REMOVAL OF TEST SUBSTANCE
- Washing: Lutrol 50%

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

animal #1

Time point:

other: 24 hours

Score:

0

Max. score:

4

Remarks on result:

other: 1, 5, and 15 minute exposure

Results 2

Irritation parameter:

edema score

Basis:

animal #1

Time point:

other: 24 hours

Score:

0

Max. score:

4

Remarks on result:

other: 1, 5, and 15 minute exposure

Results 3

Irritation parameter:

erythema score

Basis:

animal #3

Time point:

other: 48 hours

Score:

0

Max. score:

4

Remarks on result:

other: 20 hour exposure

Results 4

Irritation parameter:

edema score

Basis:

animal #3

Time point:

other: 48 hours

Score:

0

Max. score:

4

Reversibility:

other: scaling observed after 8 days

Remarks on result:

other: 20 hour exposure

Irritant / corrosive response data:

1, 5, and 15 minutes exposure: One of the two animals died after 25 minutes.
2 for 20 hours exposure: One of the two animals died after 90 minutes.

Other effects:

Necropsy of the animals that died revealed acute dilatation of the heart and congestive hyperemia and slightly edematised lung

Any other information on results incl. tables

#	sex	BW	exposure	findings
1	male	3.21 kg	1, 5, 15 min	exitus after 25 min
2	male	3.27 kg	1, 5, 15 min	no findings -> grade 0
3	male	3.12 kg	20 h	no - questionable findings -> grade 0
4	female	2.96 kg	20 h	exitus after 90 min

Applicant's summary and conclusion

Interpretation of results:

other:

Remarks:

No hints for justifiying a classification as skin irritating, but 2 of the 4 animals exposed for 1, 5, 15 min or 20 h, respectively, died during the study.