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EC number: 228-783-6 | CAS number: 6358-69-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: other routes
Administrative data
- Endpoint:
- acute toxicity: other routes
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1978
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- The Acute Intravenous Toxicity of Biological Stains, Dyes, and Other Fluorescent Substances
- Author:
- Lutty GA
- Year:
- 1 978
- Bibliographic source:
- TOXICOLOGY AND APPLIED PHARMACOLOGY 44, 225 - 249
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- - Principle of test: intravenous injection in the caudal vein to establish a LD50
- Short description of test conditions:
An intravenous LD50 was determined for several dyes with sufficient solubility and promise with regard to lethal dose. All dyes were administered in distilled water at their maximum solubility. The injections were given in the caudal vein of male Swiss-Webster mice, 25-30 g. Dose ranging was done with two mice per dose. Three doses were used for determination of the LD50, 10 mice per dose. The results were plotted on probit number of deaths vs log dose scales (Hagan, 1959). The mice were held for 14 days and then sacrificed by cervical dislocation.
- Parameters analysed / observed:
The thoracic and abdominal cavities of the mice, at least in the highest dosage group, were examined for macroscopic pathological changes. - GLP compliance:
- no
- Remarks:
- pre-GLP
Test material
- Reference substance name:
- Trisodium 8-hydroxypyrene-1,3,6-trisulphonate
- EC Number:
- 228-783-6
- EC Name:
- Trisodium 8-hydroxypyrene-1,3,6-trisulphonate
- Cas Number:
- 6358-69-6
- Molecular formula:
- C16H10O10S3.3Na
- IUPAC Name:
- trisodium 8-hydroxypyrene-1,3,6-trisulphonate
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- Swiss Webster
- Sex:
- male
Administration / exposure
- Route of administration:
- intravenous
- Vehicle:
- water
- Details on exposure:
- single application
- Doses:
- 3 doses up to maximum solubility
- No. of animals per sex per dose:
- 10
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed:
The thoracic and abdominal cavities of the mice, at least in the highest dosage group, were examined for macroscopic pathological changes.
Histopathological examination: Seven male Swiss-Webster mice were given 370 mg/kg bw (0.1 ml) of dye in distilled water via the caudal vein.
Two mice were sacrificed 2 days after injection, and the remaining five, at 14 days after injection.
Body weights were recorded on the day of injection and on the day of sacrifice. During the 14 days, the mice were observed for excretion of dye, unusual behavior, and general physical appearance.
Biopsies were performed on mice anesthetized with ether on Days 2 and 14. The inguinal, caudal, and lumbar lymph nodes were removed first, then the spleen, kidneys, and liver.
All tissues were preserved in 10% neutral-buffered formalin. The organs were weighed, formalin-wet, before trimming for paraffin processing. All sections were stained with Ehrlich’s hematoxylin and eosin (H and E). After viewing the H and E sections, McManus’ Pas and Gomori’s reticular stains were done if elaboration of lesions was necessary.
Results and discussion
Effect levels
- Key result
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 1 050 mg/kg bw
- Based on:
- test mat.
Any other information on results incl. tables
Results were only given in short tabular form:
LD50 1050 mg/kg bw
Excretion iv: 89% in urine
Growth: normal
Organ weight: normal
Injection reaction: none
Histopathological chages: reactive hyperplasia du to fighting lacerations
Observations and conclusions: Nontoxic
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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