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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Reference
Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from authoritative database and secondary source
Qualifier:
according to
Guideline:
other: "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances"
Principles of method if other than guideline:
Bioaccumulation study was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical 1,4-Bis(ethenyl)benzene. The study was performed according to other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively.
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material (IUPAC Name): 1,4-Bis(ethenyl)benzene- Common name: p-Divinylbenzene- Molecular formula: C10H10- Molecular weight: 130.189 g/mol- Smiles notation: C(c1ccc(cc1)C=C)=C- InChI: 1S/C10H10/c1-3-9-5-7-10(4-2)8-6-9/h3-8H,1-2H2 - Substance type: Organic- Physical state: Solid- Water solubility: 8.2 mg/l
Radiolabelling:
not specified
Vehicle:
yes
Details on preparation of test solutions, spiked fish food or sediment:
PREPARATION AND APPLICATION OF TEST SOLUTION - Name and concentration of vehicle: 1st Concentration area: HCO-40 500 microg/L, 2nd Concentration area: HCO-40 50 microg/L
Test organisms (species):
Cyprinus carpio
Route of exposure:
aqueous
Test type:
static
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
6 wk
Details on test conditions:
RANGE-FINDING / PRELIMINARY STUDY- Test concentrations : LC50(48h) 3.45 mg/l, Rice fish (Oryzias latipes)
Nominal and measured concentrations:
Nominal concentrations: 1st concentration area: 0.025 mg/l (25 µg/l)2nd concentration area: 0.0025 mg/l (2.5 µg/l)
Reference substance (positive control):
not specified
Lipid content:
5.1 %
Remarks on result:
other: Other details not known
Key result
Conc. / dose:
0.025 mg/L
Type:
BCF
Value:
> 206 - < 402 L/kg
Basis:
total lipid content
Time of plateau:
6 wk
Remarks on result:
other: Other details not known
Key result
Conc. / dose:
0.003 mg/L
Type:
BCF
Value:
> 229 - < 385 L/kg
Basis:
total lipid content
Time of plateau:
6 wk
Remarks on result:
other: Other details not known

Bioaccumulation test results:

[p-Divinylbenzene]

 

  

2 w

3 w

4 w

6 w

1st
concentration
area

Test substance
concentration (microg/L)

22.0

22.6

22.9

23.2

Bioconcentration
factor (L/kg)

279

402

332

268

218

257

257

206

2nd 
concentration
area

Test substance
concentration (microg/L)

2.11

2.14

2.14

2.17

Bioconcentration
factor (L/kg)

229

272

289

349

354

340

256

385

 

Validity criteria fulfilled:
not specified
Conclusions:
The bioconcentration factor (BCF value) of substance 1,4-Bis(ethenyl)benzene on Cyprinus carpio was determined to be in the range of 206-402 L/Kg at a conc. of 0.025 mg/l and 229-385 L/Kg at a conc. of 0.0025 mg/l, respectively.
Executive summary:

Bioaccumulation study was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of 1,4-Bis(ethenyl)benzene (CAS no. 105-06-6). The study was performed according to other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively.Cyprinus carpio was used as a test organism for the study. Test chemical was prepared inHCO-40.Test chemical nominal conc. used for the study was 0.025mg/land 0.0025 mg/l, respectively. Analytical method involve therecovery ratio:Test water: 94.6 %, Fish: 86.4 %, - Limit of detection: Test water: 1st concentration area; 1.2 ng/mL, 2nd concentration area; 0.12 ng/mL, Fish: 18 ng/g.Range finding study involve theLC50(48h) 3.45 mg/lonRice fish (Oryzias latipes).The bioconcentration factor (BCF value) of substance1,4-Bis(ethenyl)benzene on Cyprinus carpio was determined to bein the range of 206-402 L/Kg at a conc. of 0.025 mg/l and 229-385 L/Kg at a conc. of 0.0025 mg/l, respectively, which does not exceed the bioconcentration threshold of 2000, indicating that the chemical 1,4-Bis(ethenyl)benzene is not expected to bioaccumulate in the food chain.

Description of key information

Bioaccumulation study was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of 1,4-Bis(ethenyl)benzene (CAS no. 105-06-6) (from authoritative database (J-CHECK, 2018) and secondary source). The study was performed according to other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively.Cyprinus carpio was used as a test organism for the study. Test chemical was prepared inHCO-40.Test chemical nominal conc. used for the study was 0.025mg/land 0.0025 mg/l, respectively. Analytical method involve therecovery ratio:Test water: 94.6 %, Fish: 86.4 %, - Limit of detection: Test water: 1st concentration area; 1.2 ng/mL, 2nd concentration area; 0.12 ng/mL, Fish: 18 ng/g.Range finding study involve theLC50(48h) 3.45 mg/lonRice fish (Oryzias latipes).The bioconcentration factor (BCF value) of substance 1,4-Bis(ethenyl)benzene on Cyprinus carpio was determined to be in the range of 206-402 L/Kg at a conc. of 0.025 mg/l and 229-385 L/Kg at a conc. of 0.0025 mg/l, respectively, which does not exceed the bioconcentration threshold of 2000, indicating that the chemical 1,4-Bis(ethenyl)benzene is not expected to bioaccumulate in the food chain.

Key value for chemical safety assessment

Additional information

Experimental study and various predicted data for the target compound1,4-Bis(ethenyl)benzene (CAS No. 105-06-6) and various supporting studies for its structurally similar read across substance were reviewed for the bioaccumulation end point which are summarized as below:

 

In an experimental key study from authoritative database (J-CHECK, 2018) and secondary source,bioaccumulation experiment was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of 1,4-Bis(ethenyl)benzene (CAS no. 105-06-6). The study was performed according to other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively. Cyprinus carpio was used as a test organism for the study. Test chemical was prepared inHCO-40.Test chemical nominal conc. used for the study was 0.025mg/land 0.0025 mg/l, respectively. Analytical method involve the recovery ratio:Test water: 94.6 %, Fish: 86.4 %, - Limit of detection: Test water: 1st concentration area; 1.2 ng/mL, 2nd concentration area; 0.12 ng/mL, Fish: 18 ng/g.Range finding study involve theLC50(48h) 3.45 mg/l on Rice fish (Oryzias latipes).The bioconcentration factor (BCF value) of substance1,4-Bis(ethenyl)benzene on Cyprinus carpio was determined to be in the range of 206-402 L/Kg at a conc. of 0.025 mg/l and 229-385 L/Kg at a conc. of 0.0025 mg/l, respectively.

 

In a prediction done using the BCFBAF Program of Estimation Programs Interface (2018) was used to predict the bioconcentration factor (BCF) of test chemical 1,4-Bis(ethenyl)benzene (CAS No. 105 -06 -6). The bioconcentration factor (BCF) of 1,4-Bis(ethenyl)benzene was estimated to be 148.7 L/kg whole body w.w (at 25 deg C).

 

In an another prediction done by usingBio-concentration Factor module Bio-concentration Factor of the test substance 1,4-Bis(ethenyl)benzene (CAS no. 105 -06 -6) was estimated to be 154 at pH range 1-14, respectively.

 

For the test chemical,bioaccumulation study was conducted for estimating the BCF (bioaccumulation factor) value of test chemical (from authoritative database HSDB and PubChem, 2017). The bioaccumulation factor (BCF) value was calculated using a logKow of 3.54 and a regression-derived equation. The estimated BCF (bioaccumulation factor) value of test chemical was determined to be 100 dimensionless.

 

In a supporting study from secondary source (2017) for the test item,bioaccumulation experiment was conducted on test organism Lepomis macrochirus (Bluegill Sunfish) for 30 days for evaluating the bioconcentration factor (BCF value) of test chemical. Lepomis macrochirus (Bluegill Sunfish) was used as a test organism for the study. Test chemical nominal conc. used for the study was 0. 25mg/l. Study consisted of four continuous periods: 30–day aging, 8–day equilibration, 30–day uptake, and 14–day depuration. A dynamic bioconcentration study exposing bluegill sunfish to 14C–test substance concentration of 0.25 mg/l in constant flow–through water. During a 30-day uptake period, tissue contents of 14C-test chemical ranged from 24-64 micrograms/g for whole fish, 6-23 micrograms/g for fillet, and 25-79 micrograms/g for viscera. 14C- test chemical accumulation in the fish reached a steady- state after about day 3 of uptake. The bioconcentration factor (BCF value) of substance on Lepomis macrochirus was determined to be in the range of 110 dimensionless based on a two-compartment kinetic model. at a conc. of 0. 25 mg/l, respectively.

 

On the basis of above results for target chemical1,4-Bis(ethenyl)benzene (from authoritative and modelling databases,2018), it can be concluded that the BCF value of test substance 1,4-Bis(ethenyl)benzene ranges from 148.7 –402which does not exceed the bioconcentration threshold of 2000, indicating that the chemical 1,4-Bis(ethenyl)benzene is not expected to bioaccumulate in the food chain.