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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from authoritative database and secondary source
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance 1,4-Bis(ethenyl)benzene.
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material (IUPAC Name): 1,4-Bis(ethenyl)benzene- Molecular formula: C10H10- Molecular weight: 130.189 g/mol- Smiles notation: C(c1ccc(cc1)C=C)=C- InChI: 1S/C10H10/c1-3-9-5-7-10(4-2)8-6-9/h3-8H,1-2H2 - Substance type: Organic- Physical state: Solid
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
- Concentration of sludge: 30 mg/l
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
other: BOD and GC
Details on study design:
Improved type for a volatile substance
Parameter:
other: BOD
Value:
0
Sampling time:
28 d
Remarks on result:
other: Other details not known
Parameter:
other: GC
Value:
3
Sampling time:
28 d
Remarks on result:
other: Other details not known
Details on results:
Test substance undergoes 0 and 3% degradation by BOD and GC parameter in 28 days.
Validity criteria fulfilled:
not specified
Interpretation of results:
not readily biodegradable
Conclusions:
The percentage degradation of test substance 1,4-Bis(ethenyl)benzene was determined to be 0 and 3% by BOD and GC parameter in 28 days. Thus, based on percentage degradation, 1,4-Bis(ethenyl)benzene is considered to be not readily biodegradable in water.
Executive summary:

Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance1,4-Bis(ethenyl)benzene (CAS no. 105-06-6). Activated sludge was used as a test inoculums for the study. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance 1,4-Bis(ethenyl)benzene was determined to be 0 and 3% by BOD and GC parameter in 28 days. Thus, based on percentage degradation, 1,4-Bis(ethenyl)benzene is considered to be not readily biodegradable in nature.

Description of key information

Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance1,4-Bis(ethenyl)benzene (CAS no. 105-06-6) (from authoritative database (J-CHECK, 2018) and secondary source). Activated sludge was used as a test inoculums for the study. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance 1,4-Bis(ethenyl)benzene was determined to be 0 and 3% by BOD and GC parameter in 28 days. Thus, based on percentage degradation, 1,4-Bis(ethenyl)benzene is considered to be not readily biodegradable in nature.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

Experimental study and predicted data for the target compound 1,4-Bis(ethenyl)benzene(CAS No. 105-06-6) and various supporting studies for its structurally similar read across substance were reviewed for the biodegradation end point which are summarized as below:

 

In an experimental key study from authoritative database (J-CHECK, 2018) and secondary source, biodegradation experiment was conducted for 28 days for evaluating the percentage biodegradability of test substance1,4-Bis(ethenyl)benzene (CAS no. 105-06-6). Activated sludge was used as a test inoculums for the study. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance 1,4-Bis(ethenyl)benzene was determined to be 0 and 3% by BOD and GC parameter in 28 days. Thus, based on percentage degradation, 1,4-Bis(ethenyl)benzene is considered to be not readily biodegradable in nature.

 

In a prediction using the Estimation Programs Interface Suite (2018), the biodegradation potential of the test compound 1,4-Bis(ethenyl)benzene (CAS no. 105 -06 -6) in the presence of mixed populations of environmental microorganisms was estimated. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that chemical 1,4-Bis(ethenyl)benzene is expected to be not readily biodegradable.

 

For the test chemical from authoritative databases (2018),biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance. The study was performed according to OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I) under aerobic conditions. Activated sludge was used as a test inoculums for the study. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance was determined to be 0, 1 and 4% by BOD, TOC removal and HPLC parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.

 

In a supporting study from authoritative databases (J-CHECK, 2018 and EnviChem, 2014) for the test item,biodegradation experiment was conducted for 28 days for evaluating the percentage biodegradability of test substance. The study was performed according to OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I). Activated sludge was used as a test inoculums for the study. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance was determined to be 2 and 0% by BOD and HPLC parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.

On the basis of above results for target chemical1,4-Bis(ethenyl)benzene(from authoritative database, secondary source and modelling database, 2017), it can be concluded that the test substance 11,4-Bis(ethenyl)benzenecan be expected to be not readily biodegradable in nature.