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Toxicity to microorganisms

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Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From June 25, 2019 to September 24, 2019
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
Qualifier:
according to
Guideline:
EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
Qualifier:
according to
Guideline:
ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
GLP compliance:
yes (incl. certificate)
Specific details on test material used for the study:
Batch: SC00026940
Aspect: yellow liquid
Expiration date: July 11, 2020
Purity: 92.9 % (peak #1), 4.1 % (peak #2) and 1.3 % (alpha-Tocopherol)
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
other: synthetic sewage feed
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
3 h
Key result
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Key result
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Key result
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Validity criteria fulfilled:
yes
Conclusions:
At the tested concentration (nominal 100 mg/l) no significant inhibition was observed.
Executive summary:

An activated sludge respiration inhibition test was performed with Safranal P. In a limit test a test concentration of 100 mg/l (nominal) was tested.

At the tested concentration (nominal 100 mg/l) no significant inhibition was observed.

Therefore: EC50(3 hours) of Safranal P > 100 mg/l; EC10(3 hours) of Safranal P > 100 mg/l; NOEC (3 hours) of Safranal P = 100 mg/l

Description of key information

In an activated sludge respiration inhibition test performed according to OECD 209 guidelines, no significant inhibition was observed at the tested concentration of 100mg/L. This concentration can be considered as a NOEC for the toxicity to STP microorganisms.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
100 mg/L

Additional information

An activated sludge respiration inhibition test was performed with Safranal P. A limit test at a test concentration of 100 mg/l (nominal) was performed. No significant inhibition was observed. Therefore the EC50(3 hours) of Safranal P > 100 mg/l; EC10(3 hours) of Safranal P > 100 mg/l and the NOEC (3 hours) of Safranal P = 100 mg/l.