Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented, test procedure in accordance with official guideline, meets generally accepted scientific principles, acceptable for assessment.
according to guideline
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
not specified
GLP compliance:
not specified
Analytical monitoring:
not specified
Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna Straus were from the Stamm Berlin.
Test type:
not specified
Water media type:
Limit test:
Total exposure duration:
48 h
Test temperature:
19 - 21 °C
Dissolved oxygen:
Oxygen content 48h: 7.8 mg/l at 0 and 48 h
Molar ratio Na:K = 11.2 : 3.2
Nominal and measured concentrations:
0 and 125 mg/l
Details on test conditions:
Test vessel: Centrifuge tubes with a flattened bottomTest volume: 50 ml
Reference substance (positive control):
not specified
48 h
Dose descriptor:
Effect conc.:
> 125 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
Validity criteria fulfilled:
The results of the test showed a EC50 48 h > 125 mg/l.
Executive summary:

The result of the acute toxicity to Daphnia magna was performed according to OECD 202.

The results of the test showed a EC50 48 h > 125 mg/l.

Based on the criteria of the Regulation 1272/2008, the substance does not required to be classfied for the environment.

Description of key information

There are two available studies performed both on Acid Yellow 23 and on another form of this dye.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
125 mg/L

Additional information

The key study has been performed for different concentration and based on the active ingredient a value of EC50 > 125 mg/l has been established.

Based on the criteria of the CLP Regulation (EC n. 1272/2008), the substance does not required the classification for environment.