Registration Dossier

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Based on the results of the test performed on Acid Yellow 23, the test susbstance should be considered as not toxic for the three environmental thophic levels.

Additional information

Acute short-term administration on fish:

The assessment was based on one study performed on another form of Acid Yellow 23. The test result does not show any toxic effects on fish.

LC50 fish (96 h) > 120 mg/l

Acute short-term administration on invertebrates:

The assessment is based on two available studies both according OECD guideline 202.

One study was performed on Acid Yellow 23 and one on another form of the same colour index.

Both of the acute toxicity to Daphnia magna studies does not show any toxic effects.

EC50(48h) > 125 mg/l

Acute short-term administration on aquatic plants.

The assessment is based on two available studies both according OECD 201.

One study was performed on Acid Yellow 23 and one on another form of the same colour index.

Both of the acute toxicity to aquatic palnts studies does not show any toxic effects.

EC50(48h) > 125 mg/l


The test substance is very soluble in pure (deionised) water, none sedimentation has been observed during the tests. The substance shows a high toxicity level as shown during the observation period for the tested trophic levels.

According to the CLP Regulation (EC 1272/2008), acute aquatic toxicity is normally determined using a fish 96-hour LC50, a crustacean species 48-hour EC50 and/or an algal species 72- or 96-hour EC50; nevertheless it is also specified in the Regulation that data on other species (e. g. Lemna spp.) shall also be considered if the test methodology is suitable. These species cover a range of trophic levels and are considered as surrogate for all aquatic organisms.

According to the table 4.1.0 of the CLP Regulation (EC n. 1272/2008), a substance for wich adequate chronic toxicity data are not available shall be classified as

Aquatic Acute 1 when:

96 hr LC50 (for fish)1 mg/l and/or

48 hr EC50 (for crustacea)≤1mg/l and/or

72 or 96 hr ErC (for algae or other aquatic plants)1 mg/l

Aquatic Chronic 1 when:

96 hr LC50 (for fish) 1 mg/l and/or

48 hr EC50 (for crustacea) ≤1 mg/l and/or

72 or 96 hr ErC (for algae or other aquatic plants) 1 mg/l

Aquatic Chronic 2 when:

96 hr LC50 (for fish) > 1 <= 10 mg/l and/or

48 hr EC50 (for crustacea)> 1 <= 10 mg/l and/or

72 or 96 hr ErC (for algae or other aquatic plants)> 1 <= 10 mg/l

Aquatic Chronic 3 when:

96 hr LC50 (for fish) > 10 <= 100 mg/l and/or

48 hr EC50 (for crustacea)> 10 <= 100 mg/l and/or

72 or 96 hr ErC (for algae or other aquatic plants)> 10 <= 100 mg/l

and the substance is not rapidly degradable and/or the experimentally determined BCF ≥ 500 (or, if absent, the log K ow ≥ 4)

Taking into account the information available for the target substance, Acid Yellow 23 could be considered as not toxic for the environment.