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EC number: 923-725-2 | CAS number: -
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
Description of key information
Biofert Plusz showed a low toxicity to Eisenia fetida in a study conducted according to the guidelines OECD 222, ISO 11268-2, and BBA (28 d NOEC (mortality, body weight)=10 g test material/kg soil dry weight; 28 d LOEC (mortality, body weight)=20 g test material/kg soil dry weight; 56 d NOEC (reproduction)=2.5 g test material/kg soil dry weight; 56 d LOEC (reproduction)=10 g test material/kg soil dry weight; 56 d EC50 (reproduction)=3.23 g test material/kg soil dry weight).
Key value for chemical safety assessment
- Long-term EC10, LC10 or NOEC for soil macroorganisms:
- 2 500 mg/kg soil dw
Additional information
In a study conducted according to OECD Guideline 222 (Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei)), ISO 11268-2 (Effects of Pollutants on Earthworms. 2. Determination of Effects on Reproduction), and BBA Guideline (Guidelines for the testing of herbicides within registration progress - Effects of herbicides on reproduction and growt of Eisenia fetida/Eisenia andrei; 1994), the influence of Biofert Plusz on mortality, biomass, reproduction, behaviour and pathological symptoms of earthworms was investigated. Forty earthworms (4 replicates: 10 adults each), Eisenia fetida, were exposed to Biofert concentrations ranging from 1.25 to 20 g test material/kg soil dw for 28 d under static conditions.Thereafter, the adults were removed from the test vessels and mortality and body weight determined. After further 4 weeks the reproduction rate was determined by counting the hatched juvenils. The 28 d NOEC and LOEC based on mortality and body weight were determined to be 10 and 20 g test material/kg soil dry weight, respectively. The 56 d NOEC and LOEC based on reproduction were determined to be 2.5 and 5 g test material/kg soil dry weight, respectively. Furthermore, a 56 d EC50 (reproduction)=3.23 g test material/kg soil dry weight was found. Furthermore, no biological significant symptoms or changes in the behaviour of the earthworms were observed up to a test concentration of 10 g test material/kg soil dw after 28 d.
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