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EC number: 279-967-8 | CAS number: 82461-14-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 to 28 August 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Date of Inspection: 20 to 21 November 2018 and 7 June 2019 / date of Decision: 21 August 2019
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture:
- Name and location of sewage treatment plant where inoculum was collected: Bois-de Bay, 1242 Satigny, Switzerland
- Method of cultivation: The sludge was kept aerobic until being used.
- Preparation of inoculum for exposure: The suspended solids content was determined by drying 50 ml of the sludge and weighing. A final concentration of 1.5 g/L of suspended solid was used for inoculum as per ISO 8192-1986. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2°C
- pH:
- 8.18 - 8.42
- Dissolved oxygen:
- 4.70 - 7.90 mg/L (at beginning of the test)
- Nominal and measured concentrations:
- Nominal concentration: 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 500-ml scaled cylinders
- Type (delete if not applicable): closed
- Aeration: compressed air
- Sludge concentration (weight of dry solids per volume): 2.04 g/L
- Weight of dry solids per volume of reaction mixture per unit of time: 1.5 g/L
- Nutrients provided for bacteria: A synthetic sewage feed
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Dissolved oxygen
TEST CONCENTRATIONS
- Test concentrations: 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Validity criteria fulfilled:
- yes
- Conclusions:
- An activated sludge respiration inhibition test was performed for test substance according to OECD Guideline No. 209, ISO 8192-1986 and EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test). 3 hour EC10 and EC50 were determined to be > 100mg/L and NOEC = 100 mg/L
- Executive summary:
An activated sludge respiration inhibition test was performed for test substance according to OECD Guideline No. 209, ISO 8192-1986 and EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test).
In a limit test, a concentration of 100 mg/L (nominal) was evaluated. 3,5-dichlorophenol was used as a reference substance to check the activity of the sludge: its EC50 (3 hours) was found to be within the range 2 to 25 mg/L.
A dissolved oxygen measuring device composed of a flat bottom flask (BOD type, Karlsruher Flasche) in which an O2electrode (StirrOx G, WTW, Weilheim, Germany) fits tightly and an oxygen-meter (inolab Oxi 730, WTW, Weilheim, Germany) connected to personal computer with data recording software (Multilab®pilot v.5.21) is used to measure oxygen concentration versus time.
The pH of the sewage sludge was not measured before the test. The validity of the study is not impacted, as toxicities observed with the of the reference substance were in the expected range. The dry weight of suspended solids was 2.04 g/L. Therefore, in order to obtain the intended final suspended solids concentration of 1.5 g/L, instead of dosing 250 mL of sludge at a concentration of 3.0 g/L as stipulated in the respective standard operation procedure, 370 mL of sludge at a concentration of 2.04 g/L was dosed. The volume of water dosed was reduced accordingly. The validity of the study is not impacted, as the final concentration of sludge in the test cylinders remained unchanged.
At the tested concentration (nominal 100 mg/L) no significant inhibition was observed. Therefore, 3-hour EC10 and EC50 were determined to be > 100mg/L and NOEC = 100 mg/L.
Reference
The respiration rates were calculated from the recorder traces on a linear portion of the curve. They are expressed in mg/(l*min).
Percent inhibition was calculated as following:
% inhibition = (1 – (6Rs / RC1 + RC2 + RC3 + RC4 + RC5 + RC6)) x 100
where Rs = respiration rate at tested concentration of the test substance
RC1 = respiration rate of control C1
RC2 = respiration rate of control C2
RC3 = respiration rate of control C3
RC4 = respiration rate of control C4
RC5 = respiration rate of control C5
RC6= respiration rate of control C6.
Conditions for the validity of the test:
- The variation coefficient of the controls was 4.3%. This is within the validity range (30%).
- the EC50 (3 hours) of 3,5-dichlorophenol is between 2 and 25 mg/L.
Table 6.1.7/1: Results for test substance
Vessel |
pH |
O2[mg/I] T2.5 min |
O2[mg/I] T7.5 min |
Δ O2[mg/I] |
Time [min] |
Rate |
% Inhibition |
Concentrationfinale [mg/I] |
C1 |
8.30 |
7.00 |
5.80 |
1.20 |
5.0 |
0.24 |
- |
- |
C2 |
8.28 |
6.90 |
5.75 |
1.15 |
5.0 |
0.23 |
- |
- |
C3 |
8.29 |
6.15 |
5.05 |
1.10 |
5.0 |
0.22 |
- |
- |
S1 |
8.32 |
6.75 |
5.65 |
1.10 |
5.0 |
0.22 |
3.6 |
100.02 |
S2 |
8.18 |
5.75 |
4.70 |
1.05 |
5.0 |
0.21 |
8.0 |
100.08 |
S3 |
8.21 |
6.75 |
5.65 |
1.10 |
5.0 |
0.22 |
3.6 |
99.96 |
R1 |
8.40 |
7.55 |
6.80 |
0.75 |
5.0 |
0.15 |
34.3 |
2.04 |
R2 |
8.34 |
7.10 |
6.35 |
0.75 |
5.0 |
0.15 |
34.3 |
2.02 |
R3 |
8.37 |
6.95 |
6.30 |
0.65 |
5.0 |
0.13 |
43.1 |
2.00 |
R4 |
8.36 |
7.90 |
7.55 |
0.35 |
5.0 |
0.07 |
69.3 |
25.06 |
R5 |
8.37 |
7.85 |
7.50 |
0.35 |
5.0 |
0.07 |
69.3 |
25.16 |
R6 |
8.42 |
7.90 |
7.60 |
0.30 |
5.0 |
0.06 |
73.7 |
24.98 |
C4 |
8.39 |
7.30 |
6.20 |
1.10 |
5.0 |
0.22 |
- |
- |
C5 |
8.36 |
7.30 |
6.20 |
1.10 |
5.0 |
0.22 |
- |
- |
C6 |
8.33 |
7.20 |
6.00 |
1.20 |
5.0 |
0.24 |
- |
- |
Description of key information
OECD guideline, 209, GLP, key study, Klimish 1:
NOEC=100 mg/L (Limit test)
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
To assess the toxicity of the registered substance to microorganisms, one experimental study is available.
This valid study was performed according to OECD Guideline No. 209, with GLP statement to assess the effect of the registered on the respiration of activated sludge. In a limit test, a concentration of 100 mg/L (nominal) was evaluated. According to the results of this study, all validity criteria were fulfilled and no significant inhibition was observed at tested concentration (100 mg/L). Therefore, 3-hour EC10and EC50were determined to be > 100mg/L and NOEC = 100 mg/L.
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