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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Nov - Dec 1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: According to OECD guideline under GLP
Cross-referenceopen allclose all
Reason / purpose:
reference to same study
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report Date:
1996

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Principles of method if other than guideline:
Not relevant
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
not specified
Details on test material:
- Name of test material (as cited in study report): M-DOC
- Analytical purity: 98.2%
- Lot/batch No.: 34514021

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
5.6 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 4.1 - 7.8 mg/L

Any other information on results incl. tables

Table 1: Cumulative immobilization (%) of Daphnia magna after exposure to ZK 9340

 Concentration of ZK 9340   24 hours    48 hours  
 0,0 mg/I   5 10
 1,1 mg/I   0 15
 2,2 mg/I   0 30
 4,6 mg/I   0 30
 8,6 mg/I   15 35
 18,6 mg/I   15 100
 38,6 mg/I   100 100

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50/48 hours of ZK 9340 to Daphnia magna was calculated to be 5.6 mg/L
[95% confidence limits 4.1-7.8 mg/L]. Accordingly, ZK 9340 is toxic to Daphnia magna.
Executive summary:

The purpose of this study was to determine the acute toxicity (immobilization, EC50/48 hours) of M-DOC (ZK 9340) to Daphnia magna. 20 Daphnia were used for each of the six test concentrations of the test substance and for the dilution water control. The Daphnia were exposed to the test solutions and the dilution water for a period of 48 hours under static conditions. The test solutions were a saturated solution and 5 dilutions thereof (by a factor of 2).

Sampies for analysis by HPLC were taken at 0 and 48 hours. The concentrations of the test solutions according to analysis were 0, 1.1, 2.2, 4.6, 8.6, 18.6 and 38.6 mg/L. Immobilization was recorded at 24 hours and 48 hours. The cumulative immobilization (%) is summarized in the following table. An increasing number of immobilized Daphnia was observed in the test solutions up to 100% in a concentration of 18.6 mg/L.

The EC50/48 hours of test substance to Daphnia magna was calculated to be 5.6 mg/L [95% .confidence limits 4.1-7.8 mg/L]. Accordingly, M-DOC is toxic to Daphnia magna.