[No public or meaningful name is available]

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1974

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

FAT 40069/A was dissolved in water.

Test organisms (species):

other: Rainbow trout

Details on test organisms:

Rainbow trout: (Salmo gairdneri)
Replicates: 8 per test concentration
Age: about 4-6 months
Size: 4-8 cm
Weight: 3-6 g
Supplier: P. Hohler, 4314 Zeiningen

Further standard conditions:
- Basins have been cleaned with RBS-35 solution (FLUKA 83462) and rinsed with clean water.
- Testing water should be kept at a pH between 6 and 8.5. In case of deviations, pH needs to be adjusted with NaOH or HCl solution.
- Acclimatization period: minimum 12 days at 10-13 °C for trout.
- Feeding periods: daily (TETRA MIN, flocks, Dr. U.Baersch GmbH, D-4520 Melle, Germany)
- Day-night cycles: 12/12h
- Termination: For termination of the experiment MS-222 (Sandoz) should be used at 1% (Methacain).

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

None

Hardness:

Not available

Test temperature:

14 °C

pH:

7.4-8.0

Dissolved oxygen:

- O2-concentration must be kept at 4.8 mg/L at a minimum. Sufficient oxygen supply must be ensured. O2- target concentration 6.4 mg/L

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal concentrations: 2, 20 and 100 mg/L.

Details on test conditions:

Water:
- 15 liters for trout, Water used according to ISO guidelines.

Basins:
- Full-glass basins, 17 liters basis (36x23x26 cm) tempered (cold-room); 5 liter basins (15x20x20 cm) tempered

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

20 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

45 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- at 2 mg/L: all 8 fish where alive at 24 and 48 hours of exposure time.
- at 20 mg/L: all 8 fish where alive at 24 and 48 hours of exposure time (pH was 7.4 after 48 hours exposure time).
- at 100 mg/L: all fish died after 6 hours of exposure time (pH was 8.0 after 48 hours exposure time)

Validity criteria fulfilled:

yes

Conclusions:

The LC50 of FAT 40069/A in this fish acute toxicity test was determined to be 45 mg/L.

Executive summary:

The acute fish toxicity test was performed according to a methodology similar to OECD Guideline 203. Rainbow trout species was used in the experiment setup. Fish were tested at these different following concentrations: 2, 20 and 100 mg/L

- At 2 mg/L: all 8 fish were alive at 24 and 48 hours of exposure time.

- At 20 mg/L: all 8 fish were alive at 24 and 48 hours of exposure time (pH was 7.4 after 48 hours exposure time).

- At 100 mg/L: all fish were dead after 6 hours of exposure time (pH was 8.0 after 48 hours exposure time)

In conclusion the results obtained were.

LC0 = 20 mg/L

LC50 = 45 mg/L

LC100 =100 mg/L.

Description of key information

The LC50 of FAT 40069/A in this fish acute toxicity test was determined to be 45 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

45 mg/L

Additional information

The acute fish toxicity test was performed according to a methodology similar to OECD Guideline 203. Rainbow trout species was used in the experiment. Fish were tested at these different following concentrations: 2, 20 and 100 mg/L

- at 2 mg/L: all 8 fish were alive at 24 and 48 hours of exposure time.

- at 20 mg/L: all 8 fish were alive at 24 and 48 hours of exposure time (pH was 7.4 after 48 hours exposure time).

- at 100 mg/L: all fish were dead after 6 hours of exposure time (pH was 8.0 after 48 hours exposure time)

In conclusion the results obtained were.

LC0 = 20 mg/L

LC50 = 45 mg/L