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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1974
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
yes
Details on test solutions:
FAT 40069/A was dissolved in water.
Test organisms (species):
other: Rainbow trout
Details on test organisms:
Rainbow trout: (Salmo gairdneri)
Replicates: 8 per test concentration
Age: about 4-6 months
Size: 4-8 cm
Weight: 3-6 g
Supplier: P. Hohler, 4314 Zeiningen

Further standard conditions:
- Basins have been cleaned with RBS-35 solution (FLUKA 83462) and rinsed with clean water.
- Testing water should be kept at a pH between 6 and 8.5. In case of deviations, pH needs to be adjusted with NaOH or HCl solution.
- Acclimatization period: minimum 12 days at 10-13 °C for trout.
- Feeding periods: daily (TETRA MIN, flocks, Dr. U.Baersch GmbH, D-4520 Melle, Germany)
- Day-night cycles: 12/12h
- Termination: For termination of the experiment MS-222 (Sandoz) should be used at 1% (Methacain).
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
None
Hardness:
Not available
Test temperature:
14 °C
pH:
7.4-8.0
Dissolved oxygen:
- O2-concentration must be kept at 4.8 mg/L at a minimum. Sufficient oxygen supply must be ensured. O2- target concentration 6.4 mg/L
Salinity:
Not applicable
Nominal and measured concentrations:
Nominal concentrations: 2, 20 and 100 mg/L.
Details on test conditions:
Water:
- 15 liters for trout, Water used according to ISO guidelines.

Basins:
- Full-glass basins, 17 liters basis (36x23x26 cm) tempered (cold-room); 5 liter basins (15x20x20 cm) tempered
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
20 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
45 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC100
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- at 2 mg/L: all 8 fish where alive at 24 and 48 hours of exposure time.
- at 20 mg/L: all 8 fish where alive at 24 and 48 hours of exposure time (pH was 7.4 after 48 hours exposure time).
- at 100 mg/L: all fish died after 6 hours of exposure time (pH was 8.0 after 48 hours exposure time)
Validity criteria fulfilled:
yes
Conclusions:
The LC50 of FAT 40069/A in this fish acute toxicity test was determined to be 45 mg/L.
Executive summary:

The acute fish toxicity test was performed according to a methodology similar to OECD Guideline 203. Rainbow trout species was used in the experiment setup. Fish were tested at these different following concentrations: 2, 20 and 100 mg/L

- At 2 mg/L: all 8 fish were alive at 24 and 48 hours of exposure time.

- At 20 mg/L: all 8 fish were alive at 24 and 48 hours of exposure time (pH was 7.4 after 48 hours exposure time).

- At 100 mg/L: all fish were dead after 6 hours of exposure time (pH was 8.0 after 48 hours exposure time)

In conclusion the results obtained were.

LC0 = 20 mg/L

LC50 = 45 mg/L

LC100 =100 mg/L.

Description of key information

The LC50 of FAT 40069/A in this fish acute toxicity test was determined to be 45 mg/L.

Key value for chemical safety assessment

LC50 for freshwater fish:
45 mg/L

Additional information

The acute fish toxicity test was performed according to a methodology similar to OECD Guideline 203. Rainbow trout species was used in the experiment. Fish were tested at these different following concentrations: 2, 20 and 100 mg/L

- at 2 mg/L: all 8 fish were alive at 24 and 48 hours of exposure time.

- at 20 mg/L: all 8 fish were alive at 24 and 48 hours of exposure time (pH was 7.4 after 48 hours exposure time).

- at 100 mg/L: all fish were dead after 6 hours of exposure time (pH was 8.0 after 48 hours exposure time)

In conclusion the results obtained were.

LC0 = 20 mg/L

LC50 = 45 mg/L