Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2015
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP compliant study conducted according to OECD Guidelines, with no or minor deviations.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015
Report date:
2015

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
4-isopropylbenzonitrile
EC Number:
237-492-3
EC Name:
4-isopropylbenzonitrile
Cas Number:
13816-33-6
Molecular formula:
C10H11N
IUPAC Name:
4-(propan-2-yl)benzonitrile
Test material form:
other: liquid
Details on test material:
Batch SC00008199
Purity 99.6%
Expiry date 2015-02-26

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Details on test solutions:
6 test item concentrations in a geometric series with a separation factor of 2.2, prepared by diluting the stock solution of 10.0 mg/L with dilution water, were tested as follows:0.194 - 0.427 - 0.939 - 2.07 - 4.55 - 10.0 mg/L.

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Nominal and measured concentrations:
Nominal Concentrations: 0.194 - 0.427 - 0.939 - 2.07 - 4.55 - 10.0 mg/L.

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
9.79 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: (Cl: 4.87 – 10.0)
Duration:
24 h
Dose descriptor:
EC10
Effect conc.:
9.09 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: (Cl: 4.60 – 10.0)
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
6.13 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: (Cl: 5.26 – 7.00)
Duration:
48 h
Dose descriptor:
EC10
Effect conc.:
2.96 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: (Cl: 2.40 – 3.66)
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
2.07 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The measured concentrations of the test item at the start of the exposure intervals (0 and 24 h) were in the range of 98 to 100 % of the nominal values. At the end of the exposure intervals (24 and 48 h), the measured concentrations of the test item were in the range of 81 to 90 % of the nominal values.
The measured test item concentrations were all within ± 20 % of the nominal concentrations. This indicates that the test item concentrations were successfully maintained for the duration of the test. Therefore, the effect levels given are based on the nominal concentrations of the test item Cumin
Nitrile.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 48 h-EC50 for Daphnia magna was 6.13 mg/L (95 % confidence limits: 5.26 – 7.00 mg/L).