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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Phthalocyanine-N-[3-(diethylamino)propyl]sulfonamide copper complex
EC Number:
413-650-9
EC Name:
Phthalocyanine-N-[3-(diethylamino)propyl]sulfonamide copper complex
Cas Number:
93971-95-0
Molecular formula:
Can not be identified
IUPAC Name:
hexacopper(2+) 5-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6,14-bis({[3-(diethylamino)propyl]sulfamoyl})-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6,15-bis({[3-(diethylamino)propyl]sulfamoyl})-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 7-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 8-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
Direct dispersion in water. 200 mg of test material were dispersed in 2 L water to give a 100 mg/L stock solution from which dilutions were to give the test series.
1% Tween 80-dimethyl-sulfoxide at a concentration of 100 μL/L was added to aid dispersal of the test material.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Laboratory culture originating from a strain supplied by the Institut National de Recherche Chimique Appliquée, (I.R.CH.A), France.

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h

Test conditions

Hardness:
approximately 50mg/L as CaCO3.
Test temperature:
22 °C
pH:
7.8-7.9
Dissolved oxygen:
7.8 -8.5 mg O2/l
Nominal and measured concentrations:
1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L
Details on test conditions:
Test water : Dechlorinated (with sodium thiosulphate) and aged laboratory tap water.
Test vessels : Glass jars each containing 200 ml test solution and covered to reduce evaporation.
Experimental design : 9 test concentrations plus 1 control and 1 solvent control (including 100 μl/l of auxiliary solvent), each in duplicate. 20 animals per concentration.
Method of initiation : Daphnia were placed in the test solutions after additional of the test material.
Loading : 20 ml test solution per organism.
Photoperiod : 16h light, 8h dark.
Aeration : None.
Media renewal : None.
Criteria of effect : Daphnia were considered to be immobilised if they were unable to swim for approximately 15 seconds after gentle agitation.

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
47 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 40-56
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
26 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 22-32
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
18 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
5.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

 Cumulative Immobilisation Date for Daphnia magna Exposed for 48 Hours
    Concentration        Cumulative immobilised Daphnia               
(initial population : 10 per replicate)
    (mg/l)           24 hours           48 hours
 R1  R2  Total  %  R1  R2  Total  %
 Control  0  0  0  0  0 0  0  0
 Solvent Control  0  0  0  0  0 0  0  0
 1.0  0  0  0  0  0 0  0  0
 1.8  0  0  0  0  0 0  0  0
 3.2  0  0  0  0  0 0  0  0
 5.6  0  0  0  0  0 0  0  0
 10  0  0  0  0  1 0  1  5
 18  0  0  0  0  2  4  6  30
 32  2  1  3  15  4  5  9  45
 56  8  6  14  70  10  10  20  100
 100  10  9  19  95  10  10  20  100
                         R1 - R2 : Replicates 1 and 2.

Applicant's summary and conclusion

Conclusions:
EC₅₀=47mg/L(24h)
EC₅₀=26mg/L(48h)
No Observed Effect Concentration (NOEC) at 24h = 18 mg/L
No Observed Effect Concentration (NOEC) at 48h = 5.6 mg/L