Phthalocyanine-N-[3-(diethylamino)propyl]sulfonamide copper complex

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Phthalocyanine-N-[3-(diethylamino)propyl]sulfonamide copper complex

EC Number:

413-650-9

EC Name:

Phthalocyanine-N-[3-(diethylamino)propyl]sulfonamide copper complex

Cas Number:

93971-95-0

Molecular formula:

Can not be identified

IUPAC Name:

hexacopper(2+) 5-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6,14-bis({[3-(diethylamino)propyl]sulfamoyl})-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6,15-bis({[3-(diethylamino)propyl]sulfamoyl})-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 7-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 8-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

Direct dispersion in water. 200 mg of test material were dispersed in 2 L water to give a 100 mg/L stock solution from which dilutions were to give the test series.
1% Tween 80-dimethyl-sulfoxide at a concentration of 100 μL/L was added to aid dispersal of the test material.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Laboratory culture originating from a strain supplied by the Institut National de Recherche Chimique Appliquée, (I.R.CH.A), France.

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Hardness:

approximately 50mg/L as CaCO3.

Test temperature:

22 °C

pH:

7.8-7.9

Dissolved oxygen:

7.8 -8.5 mg O2/l

Nominal and measured concentrations:

1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L

Details on test conditions:

Test water : Dechlorinated (with sodium thiosulphate) and aged laboratory tap water.
Test vessels : Glass jars each containing 200 ml test solution and covered to reduce evaporation.
Experimental design : 9 test concentrations plus 1 control and 1 solvent control (including 100 μl/l of auxiliary solvent), each in duplicate. 20 animals per concentration.
Method of initiation : Daphnia were placed in the test solutions after additional of the test material.
Loading : 20 ml test solution per organism.
Photoperiod : 16h light, 8h dark.
Aeration : None.
Media renewal : None.
Criteria of effect : Daphnia were considered to be immobilised if they were unable to swim for approximately 15 seconds after gentle agitation.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

47 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 40-56

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

26 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 22-32

Effect concentrations 3

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

18 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

5.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Cumulative Immobilisation Date for Daphnia magna Exposed for 48 Hours     Concentration        Cumulative immobilised Daphnia                (initial population : 10 per replicate)     (mg/l)           24 hours           48 hours  R1  R2  Total  %  R1  R2  Total  %  Control  0  0  0  0  0 0  0  0  Solvent Control  0  0  0  0  0 0  0  0  1.0  0  0  0  0  0 0  0  0  1.8  0  0  0  0  0 0  0  0  3.2  0  0  0  0  0 0  0  0  5.6  0  0  0  0  0 0  0  0  10  0  0  0  0  1 0  1  5  18  0  0  0  0  2  4  6  30  32  2  1  3  15  4  5  9  45  56  8  6  14  70  10  10  20  100  100  10  9  19  95  10  10  20  100                          R1 - R2 : Replicates 1 and 2.

Applicant's summary and conclusion

Conclusions:

EC₅₀=47mg/L(24h)
EC₅₀=26mg/L(48h)
No Observed Effect Concentration (NOEC) at 24h = 18 mg/L
No Observed Effect Concentration (NOEC) at 48h = 5.6 mg/L