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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
1 (reliable without restriction)

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guideline
according to guideline
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
Principles of method if other than guideline:
Oxygen depletions in the inoculated and non-inoculated control series were within the prescribed limits*.
*Non-inoculated control : < 0.3 mg O2/L after 5 days
: < 0.4 mf O2/L after 28 days
inoculated control : < 0.5 mg O2/L after 5 days
: < 0.6 mg O2/L after 28 days
GLP compliance:

Test material

Constituent 1
Chemical structure
Reference substance name:
Phthalocyanine-N-[3-(diethylamino)propyl]sulfonamide copper complex
EC Number:
EC Name:
Phthalocyanine-N-[3-(diethylamino)propyl]sulfonamide copper complex
Cas Number:
Molecular formula:
Can not be identified
hexacopper(2+) 5-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6,14-bis({[3-(diethylamino)propyl]sulfamoyl})-2,11,20,29,37,38,39,40-octaazanonacyclo[^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6,15-bis({[3-(diethylamino)propyl]sulfamoyl})-2,11,20,29,37,38,39,40-octaazanonacyclo[^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 7-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 8-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide
Test material form:
solid: particulate/powder
migrated information: powder

Study design

Inoculum or test system:
activated sludge, domestic, adapted
Details on inoculum:
The aeration stage of the Severn Trent Plc sewage treatment plant
at Belper, Derbyshire, treating predominantly domestic sewage.

Standard culture medium
Solution 1: KH2PO4 8.5g/L, K2HPO4 21.75g/L, Na2HPO4.2H2O 33.3g/L, NH4Cl 1.7g/L pH=7.2
Solution 2: MgSO4.7H2O 22.5g/L
Solution 3: CaCl2 27.5g/L
Solution 4: FeCl3.6H2O 0.25g/L

1mL of each of solutions 1-4 were added to each litre of aerated reverse osmosis purified and deionised water.
The 'dilution water' was left at room temperature (approximately 21°C) under gentle aeration for 24 hours prior to use.
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
2 mg/L
Based on:
test mat.
Initial conc.:
3 mg/L
Based on:
other: reference substance
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
other: BOD
Details on study design:
Temperature: 21 °C
Dessolved oxygen concentrations for each test medium were determined,
in duplicate, at 0, 5, 15 and 28 days.
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

Test performance:
Mean Oxigen Depletion (mgO2/L)
Culture medium without inoculum : 0.150 (5d), 0.200 (15d), 0.275 (28d)
Culture medium with inoculum : 0.100 (5d), 0.175 (15d), 0.225 (28d)
Test material 2mg/L with inoculum : 0.550 (5d), 0.675 (15d), 0.750 (28d)
Standard material 3 mg/L with inoculum : 4.125 (5d), 4.675 (15d), 4.725 (28d)
% Degradation
% degradation (O2 consumption)
Sampling time:
28 d
Details on results:
11% degradation after 5 days
13% degradation after 15 days
15% degradation after 28 days

BOD5 / COD results

Results with reference substance:
83% degradation after 5 days
94% degradation after 15 days
95% degradation after 28 days

Applicant's summary and conclusion

Interpretation of results:
other: not ready biodegradable
The test material cannot be considered as ready biodegradable under the strict terms and conditions of OECD Guideline No.301D.