Phthalocyanine-N-[3-(diethylamino)propyl]sulfonamide copper complex

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

no

Principles of method if other than guideline:

Oxygen depletions in the inoculated and non-inoculated control series were within the prescribed limits*.
*Non-inoculated control : < 0.3 mg O2/L after 5 days
: < 0.4 mf O2/L after 28 days
inoculated control : < 0.5 mg O2/L after 5 days
: < 0.6 mg O2/L after 28 days

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Phthalocyanine-N-[3-(diethylamino)propyl]sulfonamide copper complex

EC Number:

413-650-9

EC Name:

Phthalocyanine-N-[3-(diethylamino)propyl]sulfonamide copper complex

Cas Number:

93971-95-0

Molecular formula:

Can not be identified

IUPAC Name:

hexacopper(2+) 5-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6,14-bis({[3-(diethylamino)propyl]sulfamoyl})-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6,15-bis({[3-(diethylamino)propyl]sulfamoyl})-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 7-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 8-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Study design

Inoculum or test system:

activated sludge, domestic, adapted

Details on inoculum:

The aeration stage of the Severn Trent Plc sewage treatment plant
at Belper, Derbyshire, treating predominantly domestic sewage.

Standard culture medium
Solution 1: KH2PO4 8.5g/L, K2HPO4 21.75g/L, Na2HPO4.2H2O 33.3g/L, NH4Cl 1.7g/L pH=7.2
Solution 2: MgSO4.7H2O 22.5g/L
Solution 3: CaCl2 27.5g/L
Solution 4: FeCl3.6H2O 0.25g/L

1mL of each of solutions 1-4 were added to each litre of aerated reverse osmosis purified and deionised water.
The 'dilution water' was left at room temperature (approximately 21°C) under gentle aeration for 24 hours prior to use.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

2 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

3 mg/L

Based on:

other: reference substance

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

other: BOD

Details on study design:

Temperature: 21 °C
Dessolved oxygen concentrations for each test medium were determined,
in duplicate, at 0, 5, 15 and 28 days.

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

Test performance:

Mean Oxigen Depletion (mgO2/L)
Culture medium without inoculum : 0.150 (5d), 0.200 (15d), 0.275 (28d)
Culture medium with inoculum : 0.100 (5d), 0.175 (15d), 0.225 (28d)
Test material 2mg/L with inoculum : 0.550 (5d), 0.675 (15d), 0.750 (28d)
Standard material 3 mg/L with inoculum : 4.125 (5d), 4.675 (15d), 4.725 (28d)

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

15

Sampling time:

28 d

Details on results:

11% degradation after 5 days
13% degradation after 15 days
15% degradation after 28 days

BOD5 / COD results

Results with reference substance:

83% degradation after 5 days
94% degradation after 15 days
95% degradation after 28 days

Applicant's summary and conclusion

Interpretation of results:

other: not ready biodegradable

Conclusions:

The test material cannot be considered as ready biodegradable under the strict terms and conditions of OECD Guideline No.301D.