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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
1 (reliable without restriction)

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guideline
according to guideline
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
Principles of method if other than guideline:
Oxygen depletions in the inoculated and non-inoculated control series were within the prescribed limits*.
*Non-inoculated control : < 0.3 mg O2/L after 5 days
: < 0.4 mf O2/L after 28 days
inoculated control : < 0.5 mg O2/L after 5 days
: < 0.6 mg O2/L after 28 days
GLP compliance:

Test material

Constituent 1
Chemical structure
Reference substance name:
Phthalocyanine-N-[3-(diethylamino)propyl]sulfonamide copper complex
EC Number:
EC Name:
Phthalocyanine-N-[3-(diethylamino)propyl]sulfonamide copper complex
Cas Number:
Molecular formula:
Can not be identified
hexacopper(2+) 5-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6,14-bis({[3-(diethylamino)propyl]sulfamoyl})-2,11,20,29,37,38,39,40-octaazanonacyclo[^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6,15-bis({[3-(diethylamino)propyl]sulfamoyl})-2,11,20,29,37,38,39,40-octaazanonacyclo[^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 7-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 8-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide
Test material form:
solid: particulate/powder
migrated information: powder

Study design

Inoculum or test system:
activated sludge, domestic, adapted
Details on inoculum:
The aeration stage of the Severn Trent Plc sewage treatment plant
at Belper, Derbyshire, treating predominantly domestic sewage.

Standard culture medium
Solution 1: KH2PO4 8.5g/L, K2HPO4 21.75g/L, Na2HPO4.2H2O 33.3g/L, NH4Cl 1.7g/L pH=7.2
Solution 2: MgSO4.7H2O 22.5g/L
Solution 3: CaCl2 27.5g/L
Solution 4: FeCl3.6H2O 0.25g/L

1mL of each of solutions 1-4 were added to each litre of aerated reverse osmosis purified and deionised water.
The 'dilution water' was left at room temperature (approximately 21°C) under gentle aeration for 24 hours prior to use.
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
2 mg/L
Based on:
test mat.
Initial conc.:
3 mg/L
Based on:
other: reference substance
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
other: BOD
Details on study design:
Temperature: 21 °C
Dessolved oxygen concentrations for each test medium were determined,
in duplicate, at 0, 5, 15 and 28 days.
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

Test performance:
Mean Oxigen Depletion (mgO2/L)
Culture medium without inoculum : 0.150 (5d), 0.200 (15d), 0.275 (28d)
Culture medium with inoculum : 0.100 (5d), 0.175 (15d), 0.225 (28d)
Test material 2mg/L with inoculum : 0.550 (5d), 0.675 (15d), 0.750 (28d)
Standard material 3 mg/L with inoculum : 4.125 (5d), 4.675 (15d), 4.725 (28d)
% Degradation
% degradation (O2 consumption)
Sampling time:
28 d
Details on results:
11% degradation after 5 days
13% degradation after 15 days
15% degradation after 28 days

BOD5 / COD results

Results with reference substance:
83% degradation after 5 days
94% degradation after 15 days
95% degradation after 28 days

Applicant's summary and conclusion

Interpretation of results:
other: not ready biodegradable
The test material cannot be considered as ready biodegradable under the strict terms and conditions of OECD Guideline No.301D.