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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From July 25, 2017 to October 11, 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Test organisms (species):
Daphnia magna
Details on test organisms:
- Source: István Szent University
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
245 mg/L (as CaCO3)
Test temperature:
20.6-21.0 °C
pH:
7.32-7.45
Dissolved oxygen:
7.9-8.6 mg/L
Nominal and measured concentrations:
Nominal concentrations: 100 mg/L
Details on test conditions:
- Test vessel: 50 mL glass beakers
- Test volume: ~40 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Photoperiod: 16-hr light and 8-hr dark cycle
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
The 24h-EC50: 0.64 mg/L

In Limit Test, the dissolved oxygen concentration at the end of the test was 8.5-8.6 mg O2/L. The initial pH measurements did not differ from the final measurements by more than 1.5 units.

 

Table 1. Results of the Preliminary Range-Finding Test

Nominal concentrations

(mg/L)

Untreated control

0.1

1

10

100

Number of treated / immobilished animals

10/0

10/0

10/0

10/0

10/0

Table 2. Number and percentage of immobilized animals in Limit Test

Test Group

Number of treated animals

Number of immobilized animals

24 hours

48 hours

number

percent

number

Percent

Control

20

0

0

0

0

100 mg/L

20

0

0

0

0

Validity criteria fulfilled:
yes
Conclusions:
According to OECD 202 test method, the 48-hr EC50 of CJ305 was >100 mg/L.
Executive summary:

This test using the procedures outlined in the CiToxLAB Study Plan for 16/377-023DA and OECD 202 (OECD, 2004). The results of this OECD 202 test for CJ305 show that all validity criteria were met. Based on the nominal concentrations of CJ305, the 48-hr EC50 was >100 mg/L.

Description of key information

The 48-hr EC50 of CJ305 was >100 mg/L for Daphnia magna (OECD TG202).

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information

Based on Preliminary Rang-Finding Test, a Limit Test was carried out using only one test concentration (100 mg/L) and one control group in the definitive test. In the control group for Limit Test, not more than 10 % of the Daphnia were immobilised and the dissolved oxygen concentration at the end of the test was ≥3 mgO2/L. All test validity criteria were met. The pH of the test solutions was within the ranges outlined in the OECD 202 test guideline. The measured concentrations of CJ305 were within 80-120% of their nominal concentrations. Based on the nominal concentrations of CJ305, the 48-hr EC50 was >100 mg/L.