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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): D-Milchsäureisobutylester
- Analytical purity: not reported

Test solutions

Vehicle:
not specified
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Stock solution: 2002 mg/L test substance

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
- Initial biomass concentration: 1 g/L

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 min

Test conditions

Nominal and measured concentrations:
Nominal test concentrations: 500, 1000 mg/L
Reference substance (positive control):
yes
Remarks:
3,5 Dichlorophenol

Results and discussion

Effect concentrations
Duration:
30 min
Dose descriptor:
EC0
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
EC20 (30 min) = 2 mg/L
EC50 (30 min) = 15 mg/L

Applicant's summary and conclusion