tetrasodium 3-amino-4-{[4-({4-fluoro-6-[phenyl(2-{[2-(sulfonatooxy)ethyl]sulfonyl}ethyl)amino]-1,3,5-triazin-2-yl}amino)-2-sulfonatophenyl]diazenyl}-5-hydroxynaphthalene-2,7-disulfonate

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From: Nov. 18, 2004 To: Nov. 18, 2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

soil

Radiolabelling:

no

Details on study design: HPLC method:

EQUIPMENT:
- Equipment: HPLC pump G1312A, autosampler G1313A, column oven G1316A, degaser G1322A and Dioden Array Detector G1315B by Agilent
- MOBILE PHASES: A1: 900 mL cleaned water + 100 mL KH2P04 solution (c =0.5 M), B1: acetonitrile, isocratic elution A1:B1 75:25 % (vlv)
- Flow rate: 1.0 mL/min
- Injection volume: 10 µL
- Detection wavelengths: 210 nm (dead time marker, calibration substances), 254 nm (test item)
- Injection amounts: Dead time marker: 20.4 µg, Calibration substances: 0.72 - 2.24 µg, Test item: 1.47 µg

DETERMINATION OF DEAD TIME
- Method: by inert substances which are not retained by the column
- Substance used: Formamide

CALIBRATION SUBSTANCES
- Identity: acetanilide, phenole, atrazin, monuron, triadimenol, linuron, napthalene

DETERMINATION OF RETENTION TIMES
- Test substance: The item was dissoived in distilled water and diluted 0.1 to 1 in distilled water and injected as single sample twice
- Dead time marker and reference substances: The dead time marker and the calibration substances were dissolved in acetonitrile and diluted 0.1 to 1 in distilled water and injected as single standards twice.

REPETITIONS
- Number of determinations: two

Type:

log Koc

Value:

< 1.25

Details on results (HPLC method):

- Retention times of reference substances used for calibration (mins): acetanilide (5.680 and 5.6690), phenole (5.541 and 5.543), atrazin (8.273 and 8.276), monuron (8.451 and 8.456), triadimenol (14.131 and 14.138), linuron (15.285 and 15.292) and naphthalene (16.319 and 16.332).
- Details of fitted regression line (log k' vs. log Koc): for calibration substances: test 1, log Koc = 1.55 + 1.84*logK, test 2, logKoc = 1.55 + 1.84*logK, For test substance no calculation was possible as more signals for the test item were obtained. These test conditions were not usable to determine the adsorption coefficient.

- Average retention data for test substance: test 1: 2.424 min, test 2: 2.425 min

Transformation products:

not measured

Based on the fact that the retention times of the main signal for the test substance (2.424 and 2.4250) were below the retention time of the lowest calibration substance (acetanilide with retention times of 5.680 and 5.669), it was concluded that the logK_oc value of the test item was below the lowest calibration substance (acetanilide: 1.25).

Validity criteria fulfilled:

yes

Conclusions:

Under the test conditions, the adsorption coefficient (logKoc) of the test substance was determined to be <1.25. 

Executive summary:

A study was conducted to determine the adsorption coefficient of the test substance according to the "HPLC method" described in EU Method C.19 and OECD Guideline 121, in compliance with GLP.

The test substance was injected twice into an HPLC column. A calibration graph was drawn by measuring the retention times of a series of calibration substances with known adsorption coefficients. The adsorption coefficient of the test substance was derived from its retention time and the calibration graph. Detection of the test substance was carried out at 254 nm.

For the test substance no calculation was possible as more signals for the test item were obtained. Based on the fact that the retention times of the main signal for the test substance (2.424 and 2.4250) were below the retention time of the lowest calibration substance (acetanilide with retention times of 5.680 and 5.669), it was concluded that the logK_oc value of the test item was below the lowest calibration substance (acetanilide: 1.25).

Under the test conditions, the adsorption coefficient (logK_oc) of the test substance was determined to be <1.25. 

Description of key information

Under the test conditions, the adsorption coefficient (log Koc) of the test substance was determined to be <1.25. 

Key value for chemical safety assessment

Koc at 20 °C:

17.8

Additional information

A study was conducted to determine the adsorption coefficient of the test substance according to the "HPLC method" described in EU Method C.19 and OECD Guideline 121, in compliance with GLP. The test substance was injected twice into an HPLC column. A calibration graph was drawn by measuring the retention times of a series of calibration substances with known adsorption coefficients. The adsorption coefficient of the test substance was derived from its retention time and the calibration graph. Detection of the test substance was carried out at 254 nm. For the test substance no calculation was possible as more signals for the test item were obtained. Based on the fact that the retention times of the main signal for the test substance (2.424 and 2.4250) were below the retention time of the lowest calibration substance (acetanilide with retention times of 5.680 and 5.669), it was concluded that the log K_oc value of the test item was below the lowest calibration substance (acetanilide: 1.25). Under the test conditions, the adsorption coefficient (log K_oc) of the test substance was determined to be <1.25 (Schminke, 2004b).