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EC number: 432-820-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 December 2001 to 20 December 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of relevant results.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Details on test material:
- - Name of test material (as cited in study report): S178207.
- Substance type: White powder.
- Physical state: Solid.
- Composition of test material, percentage of components: 91.4% w/w total ligand, 25.9% w/w free ligand and 69.1% w/w for the strength of S178207.
- Lot/batch No.: NBZ 0155/44.
- Storage condition of test material: At ambient temperature in the dark, in the container in which it was received.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: Aqueous samples were diluted with tetrahydrofuran and analysed. The excess test solutions were sampled at 0-h, and one replicate of the dilution water control, solvent control and each test concentration was sampled at 48-h.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution (0.18 g of the test substance in 10 ml of dimethylformamide (DMF). This stock solution was ultrasonicated for 3 minutes giving a white homogeneous suspension. The test solutions were prepared from the stock solution by addition of appropriate volumes to 1 litre of dilution water and stirring throughly. The nominal concentrations of the test solutions were 0.18, 0.32, 0.56, 1.0 and 1.8 mg/l. A solvent control was similarly prepared by the addition of 100 ul of DMF to 1 litre of dilution water, to give a solvent concentration of 100 ul/l. Equalising volumes of DMF were added, where applicable, to each of the test exposure solutions to give a final DMF concentration of 100 ul/l in each exposure vessel. All additions were made sub-surface, gradually, by microlitre syringe, into solutions vigorously stirred by magnetic follower.
- Controls:DMF and water.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): DMF for the preparation of a stock solution, and water as media for the test.
- Concentration of vehicle in test medium (stock solution): 100 ul/l.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): The resultant solutions were clear and colourless.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea.
- Source: From continuous laboratory cultures. The parental animals were from Brixham Environmental Laboratory, Brixham, Devon, UK.
- Age at study initiation (mean and range, SD): < 24 hours old, 1st instar.
- Weight at study initiation (mean and range, SD): Weight unknown.
- Breeding: The parent animals were 27 - 29 days old and had been maintained with a twoce weekly renewal of reconstituted water medium.
- Feeding during test
- Food type: Defined diet of algae Chlorella vulgaris, strain CCAP 211/12 and commercially available microencapsulated diet Frippak Booster.
ACCLIMATION
- Health during acclimation (any mortality observed): Monitored using a reference toxicant, potassium dichromate, twice a year.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- Not applicable.
Test conditions
- Hardness:
- 202.7 mg/l of CaCo3.
- Test temperature:
- 20 ± 1ºC.
- pH:
- 7.89 - 7.99.
- Dissolved oxygen:
- 8.2 - 8.6 mh/l.
- Salinity:
- Not applicable.
- Nominal and measured concentrations:
- 0.18, 0.32, 0.56, 1.0 and 1.8 mg/l (nominal), and 0.21, 0.36, 0.58, 1.0 and 1.8 mg/l (measured).
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: closed
- Material, size, headspace, fill volume: Borosilicate glass beakers of 250 ml nominal capacity.
- Aeration: No.
- Renewal rate of test solution (frequency/flow rate): Not applicable, as static test conditions.
- No. of organisms per vessel: 20.
- No. of vessels per concentration (replicates): 4.
- No. of vessels per control (replicates): Unknown.
- No. of vessels per vehicle control (replicates): Unknown.
- Biomass loading rate: 25 Daphnia/l.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt's M4 Daphnia medium.
- Particulate matter: No metal or pesticide contained.
OTHER TEST CONDITIONS
- Adjustment of pH: No.
- Photoperiod: Photoperiod of 16 hours light and 8 hours darkness with 20 minute dawn and dusk transition periods.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immbilisation - macroscopic observation of whole body movement, relative to the water, within a period of 15 seconds even if movement of individual appendages was visible.
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.65 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: moving average angle
- Remarks on result:
- other: 0.52 - 0.84 mg/l
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- There was no immobility observed in the Daphnia at the nominal concentration of 0.18 mg/l. There was no immobility observed in either the diultion water or solvent control. 24 hour EC 50 is > 1.8 mg/l (05%CI NA) based on non-statistical estimation, and 48 hour EC50 is 0.65 mg/l (95%CI 0.52 - 0.84 mg/l) based on moving average angle of Daphnia.
- Results with reference substance (positive control):
- Not applicable.
- Reported statistics and error estimates:
- Not reported.
Any other information on results incl. tables
Table Analytical results a
Nominal conc (mg/l) |
Measured conc (mg/l) |
Mean measured conc over the test durationb (mg/l) |
Mean measured conc as % of nominal | |
0 hour | 48 hour | |||
Dilution water control | < 0.041 | < 0.042 | < 0.042 | - |
solvent control | < 0.041 | < 0.042 | < 0.042 | - |
0.18 | 0.21 | 0.21 | 0.21 | 117 |
0.32 | 0.36c | 0.35d | 0.36 | 113 |
0.56 | 0.57 | 0.58 | 0.58 | 104 |
1.0 | 1.0 | 1.0 | 1.0 | 100 |
1.8 | 1.8 | 1.7 | 1.8 | 100 |
a All measurements are quoted to two significant figures and percentages are quoted to the nearest integer.
b Calculated using the arithmetic mean of the 0 - 48 hour results
c Mean of triplicate analysis (0.36, 0.35, 0.36 mg/l)
d Mean of triplicate analysis (0.34, 0.35, 0.35 mg/l)
The highest limit of detection in the study was 0.042 mg/l.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- There was no immobility observed in the Daphnia at the nominal concentration of 0.18 mg/l. Therefore 48 hour NOEC = 0.18 mg/l.
- Executive summary:
Introduction. A study was performed to assess the acute toxicity of the test material, S178207, to Daphnia magna between 18 to 20 December 2001. The method followed that described in the OECD Guidelines for Testing of Chemicals (April 1984) No 202, " Acute Immobilisation Test", also considered acceptable when compared to requirements of the relevant US Environmental Protection Agency guideline and EU guideline.
Methods: Daphnia magna, continuous laboratory cultures (parental stock 27 – 29 days old), was used for the test. The test conditions were dilution water control, solvent control and nominal concentrations of 0.18, 0.32, 0.56, 1.0 and 1.8 mg/l of the test material at 20 ± 1ºC. The duration of the study was 48 hours.
Results. The 24 hour EC 50 was > 1.8 mg/l (95%CI NA) based on non-statistical estimation, and the 48 hour EC50 was 0.65 mg/l (95%CI 0.52 - 0.84 mg/l) based on moving average angle of Daphnia. The 48 hour NOECbased on immobility was 0.18 mg/l.
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