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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
28.5. – 14.6.2001
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to
Guideline:
other: Test No. L 47 "Fish, Acute Toxicity test. Chemical formulations and waste waters."
Deviations:
yes
Remarks:
the dye is precipitated and gradually settled at the bottom
Principles of method if other than guideline:
- Principle of test:A sample of the tested substance was dissolved in the dilution water at a concentration of 0.1 g/L. This solution is initially clear, but during the test, the dye is precipitated and gradually settled at the bottom, in all dilutions.The total volume for one dilution was 1000 ml for preliminary and 4000 ml for limit test. Number of organisms: 3 (preliminary), 10 (limit test)- Short description of test conditions:The dilution water should have a pH of 7.8 and is saturated with oxygen at a temperature 20 – 24°C.Test duration 96 hours, the temperature of the solutions 22.0 ± 1.0 °C.- Parameters analysed / observed: mortality at 48 and 96 h
GLP compliance:
no
Remarks:
Zkušební laboratoř č. 1057, akr. ČIA dle ČSN EN 45001
Specific details on test material used for the study:
Other name: Saturnová námořnická modř LFG 135
Analytical monitoring:
no
Test organisms (species):
Poecilia reticulata
Details on test organisms:
TEST ORGANISM- Common name: Guppy- Strain: Poecilia reticulata- Source: AKVA-SALON, Žižkova 977, 535 01 Přelouč- Age at study initiation (mean and range, SD): 3 - 4 monts- Length at study initiation (length definition, mean, range and SD): 2 - 3 cmACCLIMATION- Acclimation period: yes- Acclimation conditions (same as test or not): same as testFEEDING DURING TESTno feeding during testfasted 72 hours before test
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Test temperature:
22.0 ± 1.0 °C (measured values in attached document with tables)
pH:
7.8 (measured values in attached document with tables)
Dissolved oxygen:
8.0 - 8.5 mg/L O2 (measured values in attached document with tables)
Nominal and measured concentrations:
initial measured concentration 100.1 mg.L-1
Details on test conditions:
TEST SYSTEM- Material, size, headspace, fill volume: open 10 L glass aquarium- Aeration: without- No. of organisms per vessel: Preliminary test: 3 animals Full test: 10 animals - No. of vessels per concentration/control (replicates): no replicatesTEST MEDIUM / WATER PARAMETERSDilution water: standard dilution water was prepared by mixing 2.5 ml of stock solutions (A,B,C,D) with 1000 ml distilled waterA) 117.6 g CaCl3.2H2O in 1L of distilled waterB) 49.3 g MgSO4.7H2O in 1L of distilled waterC) 25.9 g NaHCO3 in 1L of distilled waterD) 2.3 g KCl in 1L of distilled waterThe dilution water should have a pH of 7.8 and is saturated with oxygen at a temperature 20 – 24°C. EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality at 48, 96 h TEST CONCENTRATIONS - Range finding study: 100, 75, 50, 25 mg.L-1 - Test concentrations: 100 mg.L-1 - Results used to determine the conditions for the definitive study: see attached document with tables
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
- Results with reference substance valid? Yes
Validity criteria fulfilled:
yes
Remarks:
The measured values of pH solution not change more than 1 pH unit.
Executive summary:

The test substance, Direct Blue 85 , was tested for acute toxicity on fish Poecilia reticulata. The test was performed according to method No. L 47 "Fish, Acute Toxicity test. Chemical formulations and waste waters".

Results:

48 h - LC50 > 100.0 mg.L-1

96 h - LC50 > 100.0 mg.L-1

Description of key information

The test substance was tested for acute toxicity on fish Poecilia reticulata. The test was performed according to method No. L 47 "Fish, Acute Toxicity test. Chemical formulations and waste waters".

Results:

48 h - LC50 > 100.0 mg.L-1

96 h - LC50 > 100.0 mg.L-1

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information