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EC number: 943-172-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From October 19, 2015 to October 29, 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Details on sampling:
- Analytical samples were taken from the threshold concentration and control in 24 h intervals.
- Vehicle:
- no
- Details on test solutions:
- TEST SUBSTANCE SOLUTION PREPARATION
The necessary amount of test substance for preparing the stock solution was weighed on a weighing scoop and transferred to a volumetric flask. Test medium was added up to the bench mark and the solution was homogenised by shaking. The solution was turbid. The solution was then applied to the respective aquarium filled with test medium. Special care was taken to ensure the complete transfer of the test substance into the test vessel. The final solution in the aquarium was clear, no turbidity was observed at test start. After 48 h, the test solution appeared turbid and became more turbid each day. - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Species: Oncorhynchus mykiss Walbaum (Salmoniformes: Salmonidae)
- Source: Forellenzucht Störk, D-88348 Bad Saulgau, Germany
- Length at study initiation: 4 - 6 cm
- Feeding: each day
- Food type: granular rearing food
- Amount: 2% of bodyweight
ACCLIMATION
- Acclimation period: >12 d
- Health during acclimation: No mortality above 5% was observed throughout the acclimatisation period - Test type:
- static
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 8°dH corresponding to 143 mg CaCO3/L
- Test temperature:
- 15 to 17°C
- pH:
- 7.77 - 8.22
- Dissolved oxygen:
- The dissolved oxygen was ≥88% of air saturation.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 25 L glass aquaria
- Fill volume: 15 L
- Aeration: Continuous aeration of the test tanks with a membrane pump using a Pasteur pipette.
- No. of animals per concentration: 7 (no replicates)
- Loading: maximum loading of 1 g fish/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: water composed of dechlorinated drinking water and deionised water.
- Conductivity: 363 µS/cm
- pH of the aerated water: 6.0-8.5
- Dissolved oxygen: above 60% saturation
- Total hardness: 140-250 mg/L (as CaCO3), corresponding to 7.8-14°dH.
- Photoperiod: 12 to 16 h daily
EFFECT PARAMETERS MEASURED
Observations
Fish were observed at 0, 4, 24, 48, 72 and 96 h after test start. Fish were considered dead if there was no visible movement (e.g. gill movement), and if touching of the caudal peduncle produced no reaction. Records were made on visible abnormalities as: loss of equilibrium, swimming behaviour, respiratory function, pigmentation and all other observed events. At termination of the test, all fish were euthanized and then weighed and measured.
Measurements
Measurements of temperature, pH-value and oxygen saturation were performed in 24 h intervals. Water hardness of the untreated control was determined at the beginning of the test.
TEST CONCENTRATIONS
0 and 100 mg/L - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- In the control and at 100 mg/L, no mortality was observed within the period of the test. No sublethal effects were observed in the control and at the threshold concentration of 100 mg/L. The average weight of the test organisms was 0.94±0.18 g; the average length was 49±3 mm.
- Reported statistics and error estimates:
- Since no mortality occurred during the test, statistical evaluation for the estimation of the LC50 was not required.
- Sublethal observations / clinical signs:
The test substance is a complex mixture of various organic ingredient groups having substantially different chemical structures and the toxicity cannot be assigned to a special ingredient. Consequently, no appropriate analytical method for an accurate analysis was available. However, as approximation of the content of test substance in aqueous media, the content of total organic carbon (TOC) was measured. The content of TOC in the test substance was analysed to be 56%.
Table 2: TOC measurements during the test
Test substance nominal [mg/L]
TOC content nominal (56%)
[mg/L]Sampling
[h],TOC [mg/L]
TOC
[%]Measured in test solution
Measured in test solution (control corrected)
Control
-
0 (fresh)
0.6
-
-
-
96 (aged)
1.6
-
-
100
56.0
0 (fresh)
55.0
54.4
97
56.0
96 (aged)
54.7
53.1
95
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96 h LC50 of the test substance in Rainbow trout (Oncorhynchus mykiss ) was >100 mg/L (nominal) and the 96 h NOEC was 100 mg/L.
- Executive summary:
A study was conducted to determine the acute toxicity of the test substance in Rainbow trout (Oncorhynchus mykiss) under static conditions according to OECD Guideline 203, in compliance with GLP. The test substance was evaluated in a limit test at a threshold concentration of 100 mg/L. Test solution was prepared by dilution of the substance in medium and application of a defined volume of the stock solution to the test vessel. Assessments on effects and mortality were conducted after 0, 4, 24, 48, 72 and 96 h. Samples were taken at 0 h (initial value) and after 96 h from aged test solutions to determine total organic carbon content (TOC analysis). No sublethal effects were observed at 0 and 100 mg/L (nominal). The absence of mortality at the threshold concentration indicates that the fish is not the most sensitive group of test organism and that the LC50 is greater than the threshold concentration.TOC analysis showed that the test item was stable throughout the period of the test. The initial measured content of TOC was 98 % of the nominal concentration. The measured content of TOC in aged test solution was 95 % of the nominal concentration.Under the study conditions, the 96 h LC50 of the test substance in Rainbow trout was >100 mg/L (nominal) (Tanneberger C, 2015).
Reference
Description of key information
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
A study was conducted to determine the acute toxicity of the test substance in Rainbow trout (Oncorhynchus mykiss) under static conditions according to OECD Guideline 203, in compliance with GLP. The test substance was evaluated in a limit test at a threshold concentration of 100 mg/L. Test solution was prepared by dilution of the substance in medium and application of a defined volume of the stock solution to the test vessel. Assessments on effects and mortality were conducted after 0, 4, 24, 48, 72 and 96 h. Samples were taken at 0 h (initial value) and after 96 h from aged test solutions to determine total organic carbon content (TOC analysis). No sublethal effects were observed at 0 and 100 mg/L (nominal). The absence of mortality at the threshold concentration indicates that the fish is not the most sensitive group of test organism and that the LC50 is greater than the threshold concentration.TOC analysis showed that the test item was stable throughout the period of the test. The initial measured content of TOC was 98% of the nominal concentration. The measured content of TOC in aged test solution was 95% of the nominal concentration. Under the study conditions, the 96 h LC50 of the test substance in Rainbow trout was >100 mg/L (nominal) (Tanneberger C, 2015).
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