Trisodium 1-amino-4-[[3-[[4-chloro-6-[(sulphonatophenyl)amino]-1,3,5-triazin-2-yl]amino]-2,4,6-trimethyl-5-sulphonatophenyl]amino]-9,10-dihydro-9,10-dioxoanthracene-2-sulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1984

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

Comparable to internationally accepted guidelines.

GLP compliance:

no

Specific details on test material used for the study:

Name: FAT 41001/D
Purity: 78.8 %

Analytical monitoring:

no

Vehicle:

not specified

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

25 °C

pH:

8 - 8.2

Dissolved oxygen:

7.7 - 7.8

Nominal and measured concentrations:

100, 300 and 1000 mg/L

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

not specified

Conc. based on:

not specified

Basis for effect:

not specified

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

143.12 mg/L

Nominal / measured:

not specified

Conc. based on:

not specified

Basis for effect:

not specified

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

300 mg/L

Nominal / measured:

not specified

Conc. based on:

not specified

Basis for effect:

not specified

Details on results:

After 48h: 10/10, 0/10, 0/10 fishes survived at 100 mg/L, 300 mg/L and 1000 mg/L respectively.
After 96h: 10/10, 0/10, 0/10 fishes survived at 100 mg/L, 300 mg/L and 1000 mg/L respectively.

Validity criteria fulfilled:

not specified

Conclusions:

The 96h LC50 of FAT 41001/D in this fish acute toxicity test was determined to be 143.12 mg/L.

Executive summary:

The acute fish toxicity test of FAT 41001/D was performed using a methodology equivalent or similar to OECD Guideline 203. 10 Zebra fish per concentration were exposed to FAT 41001/D for 96 hours at following concentrations: 100, 300 and 1000 mg/L.

After 48h: 10/10, 0/10, 0/10 fishes survived at 100 mg/L, 300 mg/L and 1000 mg/L respectively.

After 96h: 10/10, 0/10, 0/10 fishes survived at 100 mg/L, 300 mg/L and 1000 mg/L respectively.

Based on these findings, the 96h LC50 of FAT 41001/D in this fish acute toxicity test was determined to be 143.12 mg/L.

Summary;

LC0 (48 h) : 100 mg/L

LC50 (48 h) : 181.5 mg/L

LC100 (48 h) : 300 mg/L

LC0 (96 h) : 100 mg/L

LC50 (96 h) : 143.12 mg/L

LC100 (96 h) : 300 mg/L

Description of key information

Six studies were conducted to determine the acute toxicity of FAT 41001 to zebra fish (Danio rerio). The lowest 96h  LC50 value found was 143 mg/L. The most recent study conducted to determine the acute toxicity of FAT 41001 to zebra fish (Danio rerio) was performed in 1984. Groups of 10 fish were exposed at 300 or 100 mg/L for a duration of 96 h. The number of surviving fish was recorded at 1, 4, 24, 48, 72 and 96 h. Mortality was observed only at the high dose, with one fish dying within 4 h. Under the test conditions, the 96 h LC0 was 100 mg/L, whereas the 96 h LC50 was >143 mg/L and LC100 was 300 mg/L. Few more studies performed in 1974, 1982, 1983 & 1984 reported LC50 (48 h & 96 h) to zebra fish as being in the same order of magnitude or higher. In conclusion, the test item FAT 41001 had no acute toxic effects on zebra fish under the present test conditions.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

143 mg/L