Sodium 3-[[3-methoxy-4-[(4-methoxyphenyl)azo]phenyl]azo]benzenesulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Study completion date - 18 April 1978.

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

Internal method A-11

Deviations:

no

GLP compliance:

no

Specific details on test material used for the study:

Name: FAT 20004/D
Purity: 61 %

Analytical monitoring:

no

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

14 °C

pH:

7.9-8.3

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

other: TL0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

other: TL50

Effect conc.:

140 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

other: TL100

Effect conc.:

200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- At 100 mg/L: all 8 fishes were alive at 24 and 48 hours of exposure time.
- At 200 mg/L: all 8 fishes were dead at 24 hours of exposure time.

Validity criteria fulfilled:

no

Conclusions:

The TL50 of FAT 20004/D in this fish acute toxicity test was determined to be 140 mg/L.

Executive summary:

The acute fish toxicity test was performed according internal method similar to current OECD guideline 203 using freshwater fish, Oncorhynchus mykiss.

Fishes were tested at these following different concentrations: 100, 200 & 1000 mg/L

- At 100 mg/L: all 8 fishes were alive at 24 and 48 hours of exposure time (pH was 8.2 after 48 hours exposure time).

- At 200 mg/L: all 8 fishes were dead at 24 hours of exposure time (pH was 8.3 after 48 hours exposure time).

 

In conclusion:

TL0 = 100 mg/L

TL50 = 140 mg/L

TL100 = 200 mg/L.

Description of key information

Several tests were performed in the 1970’s to access toxicity of the test substance FAT 20004 in the fish. The toxicity test was performed according internal method similar to current OECD guideline 203 using freshwater fish, Oncorhynchus mykiss. The lowest TL50 determined was 140 mg/l and the highest recorded was 450 mg/l. Hence, it can be concluded that TL50 was >100 mg/l.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

140 mg/L