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Diss Factsheets
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EC number: 207-542-9 | CAS number: 480-16-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 438 (Isolated Chicken Eye Test Method for Identifying Ocular Corrosives and Severe Irritants)
- Qualifier:
- according to guideline
- Guideline:
- EU method B.48 (Isolated chicken eye test method for identifying occular corrosives and severe irritants)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2',3,4',5,7-pentahydroxyflavone
- EC Number:
- 207-542-9
- EC Name:
- 2',3,4',5,7-pentahydroxyflavone
- Cas Number:
- 480-16-0
- Molecular formula:
- C15H10O7
- IUPAC Name:
- 2',3,4',5,7-pentahydroxyflavone
- Reference substance name:
- Morin
- IUPAC Name:
- Morin
Constituent 1
Constituent 2
Test system
- Vehicle:
- unchanged (no vehicle)
- Amount / concentration applied:
- 30 mg/ eye
- Duration of treatment / exposure:
- 10 s
- Observation period (in vivo):
- 240 min
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- other: Corneal opacity score
- Basis:
- mean
- Time point:
- other: 240 min
- Score:
- 2
- Remarks on result:
- other: Value: 1.3
- Irritation parameter:
- other: Corneal swelling
- Basis:
- mean
- Time point:
- other: 75 min
- Score:
- 3
- Remarks on result:
- other: Value: 24 %
- Irritation parameter:
- other: Corneal swelling
- Basis:
- mean
- Time point:
- other: 240 min
- Score:
- 3
- Remarks on result:
- other: Value: 27 %
- Irritation parameter:
- other: Fluorescein retention
- Basis:
- mean
- Time point:
- other: 30 min
- Score:
- 2
- Remarks on result:
- other: Value: 0.8
Applicant's summary and conclusion
- Interpretation of results:
- other: No prediction can be made
- Remarks:
- Criteria used for interpretation of results: OECD GHS
- Conclusions:
- According to the guideline OECD 438, test item has been categorized as “No prediction can be made”.
- Executive summary:
The purpose of this Isolated Chicken Eye Test (ICET) was to evaluate the potential ocular corrosivity and irritancy of the test item Morin by its ability to induce toxicity in enucleated chicken eyes. The test compound was applied in a single dose (30 mg/eye) onto the cornea of isolated chicken eyes in order to potentially classify the test compound as either 1: causing "serious eye damage" (category 1 of the Globally Harmonised System for the Classification and Labelling of chemicals (GHS)), or 2: not requiring classification for eye irritation or serious eye damage according to the GHS. Tested corneas were evaluated pre-treatment and at approximately 30, 75, 120, 180 and 240 minutes after the post-treatment rinse. The endpoints evaluated were corneal opacity, swelling, fluorescein retention, and morphological effects. All of the endpoints, with the exception of fluorescein retention (which was determined only at pre-treatment and 30 minutes after test substance exposure) were determined at each of the above time points.
The Morin and Imidazole (positive control) were ground before use in the study. They were applied in an amount of 0.03 g/eye by powdering the entire surface of the cornea attempting to cover the cornea surface uniformly with the test substance or positive control. Three test item treated eyes and three positive control eyes were used in this study.
One negative control eye was treated with 30 μL saline solution.
After an exposure period of 10 seconds from the end of the application the cornea surface was rinsed thoroughly with ~20 mL saline solution at ambient temperature and this procedure was repeated for each eye.
In this ICET, Morin did not cause ocular corrosion or severe irritation in the enucleated chicken eyes. The overall ICE class was 2×II, 1xIII. Positive and negative controls showed the expected results.
According to the guideline OECD 438, Morin overall in vitro classification is neither UN GHS Classification Category I (an ocular corrosive or severe eye irritant) nor No Category. Thus, according to the guideline OECD 438, test item has been categorized as “No prediction can be made”.
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