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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From Februry 11, 2016 to March 24, 2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reference:
Composition 1
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Test material information:
Composition 1
Test organisms (species):
Daphnia magna
Details on test organisms:
- Source: Aquatic BioSystems (Fort Collins, CO)
Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Hardness:
Hardness of 100 mg/L as CaCO3
Test temperature:
19.6-20.6°C
pH:
7.9-8.3
Dissolved oxygen:
9.0-9.1mg/L
Nominal and measured concentrations:
Nominal Concentrations of the Test Item were 6.25, 12.5, 25, 50 and 100 mg/L
Details on test conditions:
- Test vessel: ~250 mL glass beakers
- Test volume: 200 mL
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Photoperiod: 16-hr light: 8-hr dark with a 30 minute transition period
- Light intensity: 687-773 lux
Reference substance (positive control):
yes
Remarks:
Zinc sulphate heptahydrate (CAS No. 7446-20-0)
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
70.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
48-hr LC50: 0.32 mg Zn2+/L

The 0% of control organisms were immobilised and the dissolved oxygen concentration at the end of the test was 9.0-9.1 mgO2/L. The initial pH measurements did not differ from the final measurements by more than 1.5 units in each test. The color of the test solutions remained the same throughout the test.

Table 1. Number and Percentage of Daphnia Immobilised after 24-hr Exposure

Nominal Concentration of Test Item

(mg/L)

Mean ± SD

(%)

Replicate A

(# / %)

Replicate B

(# / %)

Replicate C

(# / %)

Replicate D

(# / %)

0 (Neg./Lab. Control)

0 ± 0

0 / 0

0 / 0

0 / 0

0 / 0

6.25

0 ± 0

0 / 0

0 / 0

0 / 0

0 / 0

12.5

0 ± 0

0 / 0

0 / 0

0 / 0

0 / 0

25

0 ± 0

0 / 0

0 / 0

0 / 0

0 / 0

50

0 ± 0

0 / 0

0 / 0

0 / 0

0 / 0

100

0 ± 0

0 / 0

0 / 0

0 / 0

0 / 0

 

Table 2. Number and Percentage of Daphnia Immobilised after 48-hr Exposure

Nominal Concentration of Test Item

(mg/L)

Mean ± SD

(%)

Replicate A

(# / %)

Replicate B

(# / %)

Replicate C

(# / %)

Replicate D

(# / %)

0 (Neg./Lab. Control)

0 ± 0

0 / 0

0 / 0

0 / 0

0 / 0

6.25

0 ± 0

0 / 0

0 / 0

0 / 0

0 / 0

12.5

0 ± 0

0 / 0

0 / 0

0 / 0

0 / 0

25

0 ± 0

0 / 0

0 / 0

0 / 0

0 / 0

50

0 ± 0

0 / 0

0 / 0

0 / 0

0 / 0

100

100 ± 0

5 / 100

5 / 100

5 / 100

5 / 100

Table 3.Test Endpoint Summary for CJ304

Concentration

(mg/L)

CJ304 (mg/L)

48-hr EC50

LOEC

NOEC

Nominal

70.7

100

50

Validity criteria fulfilled:
yes
Conclusions:
According to OECD 202 test method, the 48-hr EC50 of CJ304 was 70.7 mg/L.
Executive summary:

This test using the procedures outlined in the Maxxam Study Plan for TOX1605 which is based on the SOP for the OECD 202 (BBY2SOP-00017) and OECD 202 (OECD, 2004). The results of this OECD 202 test for CJ304 show that all validity criteria were met.

Based on the nominal concentrations of CJ304:

1.     The 48-hr EC50 was 70.7 mg/L.

2.     The NOEC and LOEC was 50 mg/L and 100 mg/L, respectively.

Description of key information

The 48-hr EC50 of CJ304 was 70.7 mg/L for Daphnia magna (OECD TG202).

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
70.7 mg/L

Additional information

The test was considered valid as 0% of control organisms were immobilised and the minimum dissolved oxygen concentration at the end of the test was 9.0-9.1 mg O2/L. All test validity criteria were met. The pH of the test solutions was within the ranges outlined in the OECD 202 test guideline. The measured concentrations of CJ304 were within 80-120% of their nominal concentrations.

Based on the nominal concentrations of CJ304:

1.     The 48-hr EC50 was 70.7 mg/L.

2.     The NOEC and LOEC was 50 mg/L and 100 mg/L, respectively.