2,4-dichloroaniline

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

other: BUA report

Adequacy of study:

other information

Reliability:

other: BUA report

Rationale for reliability incl. deficiencies:

other: No reliability is given as this is a summary entry for the BUA report.

Data source

Reference

Reference 1

Reference Type:

secondary source

Title:

2,4-Dichloranilin; 2,5-Dichloranilin; 3,4-Dichloranilin

Author:

Beratergremium für umweltrelevante Altstoffe (BUA)

Year:

1994

Bibliographic source:

Wissenschaftliche Verlagsgesellschaft, BUA report 140

Reference 2

Reference Type:

other: dissertation

Title:

Toxizitätsbestimmung von umweltrelevanten Chemikalien mit einem neuen Biotestsystem, Ermittlung physikochemischer Eigenschaften und Ableitung quantitativer Struktur-Toxizitäts-Beziehungen unter Anwendung von Multiregressions- und Hauptkomponenten-Analyse.

Author:

Nendza, M.

Year:

1987

Bibliographic source:

Dissertation, 42-65, 106-107, 138-143

Reference 3

Reference Type:

publication

Title:

Quantitative structure-toxicity relationships and multivariate data analysis for ecotoxic chemicals in different biotestsystems.

Author:

Nendza, M. and Seydel, J. K.

Year:

1988

Bibliographic source:

Chemosphere 17, 1575-1584

Reference 4

Reference Type:

publication

Title:

Toxicities of chloroanilines to Photobacterium phosphoreum and their correlations with effects on other organisms and structural parameters.

Author:

Ribo, J. M. and Kaiser, K. L. E.

Year:

1984

Bibliographic source:

QSAR in Environmental Toxicology, pp 319-336

Reference 5

Reference Type:

publication

Title:

Toxicities of selected chloroanilines to four strains of yeast.

Author:

Kwasniewska, K. and Kaiser, K. L. E.

Year:

1984

Bibliographic source:

QSAR in Environmental Toxicology, pp 223-233

Reference 6

Reference Type:

publication

Title:

Hefe als biologisches Testsystem für ökotoxikologische Untersuchungen. [in German]

Author:

Gies A., Pauli W., Benzing M.

Year:

1989

Bibliographic source:

UBA, Chemikaliengesetz Heft 8, Prüfung und Bewertung von Stoffen auf ihre Umweltgefährlichkeit, pp1-2, 153-164, 213

Reference 7

Reference Type:

publication

Title:

Schadwirkungen von Umweltchemikalien an biologischen Membranen [in German]

Author:

Cascorbi I, Roesick E, Wiegener B, Gies A

Year:

1989

Bibliographic source:

In: Umweltbundesamt (ed.): Chemikaliengesetz Heft 8, Pruefung und Bewertung von Stoffen auf ihre Umweltgefaehrlichkeit, S. 165-178, 210-211, Berlin 1989

Materials and methods

Principles of method if other than guideline:

BUA report

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2,4-dichloroaniline

EC Number:

209-057-8

EC Name:

2,4-dichloroaniline

Cas Number:

554-00-7

Molecular formula:

C6H5Cl2N

IUPAC Name:

2,4-dichloroaniline

Results and discussion

Any other information on results incl. tables

Tests on bacteria

test organism	Temperature (°C)	test system	exposure	result	source
Escherichia coli	37	=> static	=> exponential growth phase => first measurement 45 minutes after addition of test substance	IC50 = 62 mg/l	Nendza (1987), Nendza and Seydel (1988)
Photobacterium phosphoreum (marine)	15	=> static => inhibition of bioluminescence (Microtox test)	5 min	IC50 = 4.0 mg/l	Ribo and Kaiser (1984)
			15 min	IC50 = 4.6 mg/l
			30 min	IC50 = 4.7 mg/l

 

Toxicity of 2,4-dichloroaniline on cell proliferation and transport capacity of the cell membrane of yeast (no indication of temperature)

test organism	Basis for effect	measured parameters	exposure	result	source
Rhodotorula rubra (Origin: the shores of,)	cell proliferation	cell density	24 h	IC50 = 18 mg/l	Kwasniewska and Kaiser (1984)*
Rhodotorula spec. (origin: activated sludge of a municipal STP)				IC50 = 20 mg/l
Pichia spec.				IC50 = 60 mg/l
Saccharomyces cerevisiae(origin: commercial product)				IC50 = 117 mg/l
Saccharomyces cerevisiae	cell proliferation	cell density	24 h	IC20 = 144 mg/l IC50 = 191 mg/l IC90 = 258 mg/l	Gies et al. (1989)
		multiplication rate	exponential growth phase	IC20 = 83 mg/l IC50 = 185 mg/l IC90 = 356 mg/l
	electrical properties of the cell membrane	proportion of rotating cells in the electric field at 300 Hertz, in vivo	1.5 h	IC20 = 292 mg/l IC50 = 486 mg/l IC90 = 616 mg/l**
	transport of purine through the cell membrane into the cell	initial uptake rate of radiolabelled hypoxanthine, in vivo	10 min	IC20 = 178 mg/l IC50 = 373 mg/l IC90 = 648 mg/l**	Cascorbi et al., 1989
	proton transport through the cell membrane	ATPase activity in a cell membrane fraction, in vitro	no data	IC20 = 141 mg/l IC50 = 259 mg/l IC90 = 437 mg/l

* 2,4-dichloroaniline was predissolved in dimethyl sulfoxide

** values are above water solubility of 2,4-dichloroaniline (ca. 500 mg/l)