Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 242-895-2 | CAS number: 19224-29-4
The corneal irritation and damage potential of the test article (Viscous liquid, Lot # 565093, Purity: 91.4%) was tested in the Bovine Corneal Opacity and Permeability test (BCOP). The study was performed in compliance with EPA GLP 40 CFR 160 and 792, FDA GLP 21 CFR 58, and OECD GLP (1997). The test method was based on OECD Guideline 437 (2013). Corneas (3/group) were prepared in cell culture media. The corneas were mounted in specially designed holders and incubated at 32 C for at least 1 hour prior to exposure. Corneas were treated with 0.75 mL of the test article or appropriate control (Positive: 100% ethanol, Negative: MEM) using the closed chamber method and incubated for an approximate 10 minute exposure period at 32 C. At the end of the exposure period, the corneas were rinsed with cell culture media and incubated for 2 hours in cell culture solution. Opacity was evaluated prior to treatment and immediately following the 2 hour incubation. Immediately following the opacity measurement, the cell culture media was replaced with 0.4% sodium fluorescein solution in Dulbecco’s Phosphate Buffered Saline (DPBS) and incubated for another 90 minutes. Following the 90 minute incubation, permeability was evaluated using a fluorescein method. The mean in vitro irritation score (IVIS) was calculated and the scores were classified according to protocol defined categories. For the test article, the IVIS= 1.21. The corrected mean opacity score was 1.00 (range -1 to 1), and the mean permeability score was 0.014 (range 0.002 to 0.02). Controls performed as expected. Based on the results of the test, the test article is expected to cause no significant irritation to the cornea (IVIS = 1.21).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Close Do not show this message again