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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
26-05-2002 to 27-05-2002
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study (OECD), validity criteria fulfilled

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report date:
2002

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
L-2-hydroxy-2-phenylacetic acid
EC Number:
210-276-6
EC Name:
L-2-hydroxy-2-phenylacetic acid
Cas Number:
611-71-2
Molecular formula:
C8H8O3
IUPAC Name:
hydroxy(phenyl)acetic acid
Details on test material:
ChiPros (R)-Mandelsäure: (R)-mandelic acid (purity: 100 %; impurities: 0.1 % mandelic acid amide)

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
- preparation of inoculum: washed, conc. of dry matter: 5 g/l, aerated during the night

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
30 min

Test conditions

Dissolved oxygen:
- oxygen concentration during aeration: > 2.5 mg/l
- oxygen concentration immediatley before measurement: > 6.5 mg/l
- duration of measurement of oxygen consumption: 8-10 min

Details on test conditions:
- test vessel: Erlenmeyer flask (250 ml)
- test volume: 250 ml

- test assays: blank control (3x), reference substance (3x) and test substance (1000 mg/l)
- synthetic medium: 8 ml (100-fold concentrated OECD medium)
- inoculum: 50 ml (final conc. of dry matter: 1 g/l)
- dilution water: deionised water

Results and discussion

Effect concentrations
Duration:
30 min
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Results with reference substance (positive control):
- reference substance: 3,5-dichlorophenol; EC50 is in the range of 5-30 mg/l

Any other information on results incl. tables

effect value (30 min, mg/l)
EC20: > 1000 mg/l

parameter BC (average) TS RS1 RS2 RS3
oxygen concentration (mg/l)
start 6.8-7.8 6.9 6.9 8.1 8.6
6 min 5.0-6.5 5.0 5.0 7.1 8.3

oxygen consumption rate
(mg O2/l/h) 18 19 19 10 3

specific oxygen consumption rate
(mg O2/l/h) 18 19 19 10 3

change in oxygen consumption rate compared to BC
(%) - -6 -6 44 83

pH
before addition of inoculum (corrected)
7.1 7.6 7.2 7.3 7.0
after test end (30 min)
- 7.8 - - -
BC = blank control
RS = reference substance (reference substance conc.: RS1: 1 mg/l; RS2: 10 mg/l; RS3: 100 mg/l)
TS = test substance

effect values for reference substance (30 min, mg/l):
EC20 ca. 3.3
EC50 ca. 15
EC80 ca. 85

Applicant's summary and conclusion