Ammonium (xylenes and 4-ethylbenzene)sulfonates

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

March 29 - April 17, 1979

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

Deviations:

not specified

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Ammonium (xylenes and 4-ethylbenzene) sulphonate

EC Number:

943-024-5

Molecular formula:

C8H13NO3S

IUPAC Name:

Ammonium (xylenes and 4-ethylbenzene) sulphonate

Test animals

Species:

rat

Strain:

other: COX-SD

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS- Source: Lab Supply Company- Age at study initiation: no data- Weight at study initiation: 195 to 231 grams- Fasting period before study: overnight- Housing: individually in metal, wire-bottomed cages elevated above the droppings- Diet (e.g. ad libitum): ad libitum- Water (e.g. ad libitum): ad libitum- Acclimation period: 7 days minimumENVIRONMENTAL CONDITIONS- Temperature (°C): no data- Humidity (%): no data- Air changes (per hr): no data - Photoperiod (hrs dark / hrs light): no data IN-LIFE DATES: From: March 29, 1979 To: April 17, 1979

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

VEHICLE- Concentration in vehicle: no vehicleMAXIMUM DOSE VOLUME APPLIED: no dataDOSAGE PREPARATION (if unusual): no dataCLASS METHOD (if applicable)- Rationale for the selection of the starting dose: no data

Doses:

4.75 grams and 5.25 grams per kilogram of body weight

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days - Frequency of observations and weighing: observed on day of dosing and daily (5 days per week) for 14 days; weighed on day of dosing and at 7 days post treatment- Necropsy of survivors performed: yes- Other examinations performed: clinical signs including hypoactivity, hypersalivation, diarrhea and diuresis, plus body weight

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 250 mg/kg bw

Based on:

test mat.

Mortality:

none observed

Clinical signs:

other: Nine of the animals (6 at 4.75 g/kg bw and 3 at 5.25 g/kg bw) showed slight to moderate hypoactivity, hypersalivation, diarrhe and diuresis. All nine returned to normal between 24 and 48 hours following dosage.

Gross pathology:

Tissues were not remarkable in any of the animals at sacrifice.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

The LD50 to male and female rats was greater than 5250 mg/kg body weight.

Executive summary:

The acute Oral Toxicity of Ammonium (xylenes and 4-ethylbenzene) sulfonates was assessed following official method OECD 423, Acute Toxic Class Method. The limit test was performed with a single dose of 5250 mg/kg bw based on test material administered to both males and females Wistar rats.

Nine of the animals (6 at 4.75 g/kg bw and 3 at 5.25 g/kg bw) showed slight to moderate hypoactivity, hypersalivation, diarrhe and diuresis. All effects were reversible between 24 and 48 hours following dosage.

Dissection of animals at the end of the experiment showed no macroscopically visible organ changes.