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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Description of key information

The acute toxicity of Arbinol B in Daphnia magna was studied according to OECD 202 Guideline

6-24 hour old Daphnia magna were exposed for 48 hours to Arbinol B under static conditions at nominal concentrations of 0, 50, 87, 158, 281 and 500 mg/L. The number of dead daphnids was recorded 24 and 48 hours after exposure.

 

At or above a concentration level of 87 mg/l, white precipitates were observed at the bottom of the test vessels. Daphnia showed a white line on their back at these concentration levels. Therefore the NOEC was determined to be 50 mg/l.

At test termination, no mortality had occur among organisms exposed to the highest level tested. Thus, the 24 hour and 48 hour EC50 was higher than 500 mg/l indicating that the test substance does not exhibit toxicity to aquatic invrtebrates as per the CLP criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
500 mg/L

Additional information

No. of studies of target substance denatonium benzoate (CAS No 3734-33-6)reviewed for Short-term toxicity to aquatic invertebrates from reliable sources having Klimisch rating 4.

The summary of the results are presented below

Sr.No

Endpoint

Effect values

Basis for effects

Duration

Species

Sources

1

LC50

>500 mg/L

Mortality

24 hrs.

Daphnia magna

Study report

LC50

>500 mg/L

Mortality

48 hrs.

NOEC

50 mg/L

Appearance of a white line on their back

48 hrs.

2

LC50

400 mg/L

Mortality

96 hrs.

Crangon sp.

Study report
 

 

By applying weight of evidence approach to the target substancedenatonium benzoate, the endpoint value was found to vary between LC50= 400 to >500 mg/l in a 24, 48 & 96 Hours respectively. Study on the basis of mortality effect. But the BCF value of target substance 1denatonium benzoate, reported was not >= 500 as well as the substance was found to be rapidly degradable. Thus considering the CLP Criteria for aquatic classification of the substance, it is concluded that substance does not exhibit short term toxicity to aquatic invertebrate (Daphnia magna).