2-{2-[(2-ethylhexyl)oxy]ethoxy}ethyl prop-2-enoate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

The study was conducted between 11 August 2015 and 08 September 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: 1= Reliable without restriction o GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-{2-[(2-ethylhexyl)oxy]ethoxy}ethyl prop-2-enoate

Cas Number:

117646-83-0

Molecular formula:

C15H28O4

IUPAC Name:

2-{2-[(2-ethylhexyl)oxy]ethoxy}ethyl prop-2-enoate

Test material form:

other: liquid

Details on test material:

Identification:
M120

Batch:
NC-5635-01

Purity:
99.4%

Physical state/Appearance:
Clear colorless liquid

Expiry Date:
05 October 2015

Storage Conditions:
Room temperature, in the dark

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

6.5 mg/L

Conc. based on:

test mat.

Any other information on results incl. tables

Range Finding Test

No immobilization was observed at the test concentrations of 0.10 and 1.0% v/v saturated solution, however, immobilization was observed at 10 and 100% v/v saturated solution.

 

Based on this information test concentrations of 2.6, 6.4, 16, 40 and 100% v/v saturated solution were selected for the definitive test.

 

Chemical analysis of the test preparations at 0 hours (see Appendix 6) showed measured test concentrations to range from less than the LOQ to 20.4 mg/L. Analysis at 48 hours showed measured test concentrations to range from 0.226 to 22.7 mg/L. There was no significant change in the measured concentrations at 48 hours indicating that the test item was stable under test conditions.

  

Definitive Test

Varification of test Concentrations

Analysis of the test preparations at 0 hours (see Appendix 6) showed measured test concentrations to range from 3.38 to 21.7 mg/L. There was no significant change in the measured concentrations at 48 hours and so the results are based on 0-Hour measured test concentrations only.

  

Immobilization Data

Analysis of the immobilization data by Probit analysis using Linear Maximum-Likelihood regression 0-Hour measured test concentrations gave the following results:

Time (h)	EC50(mg/L)
24	6.7
48	6.5

 

The No Observed Effect Concentration after 24 and 48 hours exposure was 3.4 mg/L. The Lowest Observed Effect Concentration after 24 and 48 hours exposure was 7.6 mg/L.

The slopes and their standard errors of the response curves at 24 and 48 hours were
1.16 (SE = 0.25) and 1.13 (SE = 1.0) respectively. Due to the nature of the data it was not possible to calculate the 95% Confidence Limits at 24 and 48 hours.

  

Sub-Lethal Effects

No sub-lethal effects of exposure were observed throughout the test.

  

Validation Criteria

The test was considered to be valid given that none of the control daphnids showed immobilization or other signs of disease or stress and that the oxygen concentration at the end of the test was equal to or greater than 3 mg/L in the control and test vessels.

  

Water Quality Criteria

Temperature was maintained at approximately 21 °C throughout the test, while there were no treatment related differences for oxygen concentration or pH.

 

Throughout the test the light intensity was observed to be in the range 649 to 671 Lux.

  

Observations on Test Item Solubility

At the start and throughout the test all control and test solutions were observed to be clear colorless solutions.

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Exposure of Daphnia magna to the test item has been investigated and gave the following results based on the 0-Hour measured test concentrations:
EC50 = 6.5 mg/L
NOEC = 3.4 mg/L
LOEC = 7.6 mg/L