Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 282-468-8 | CAS number: 84229-70-9
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
The test substance showed a low acute toxicity to Daphnia magna with an EC50 of 748 mg/L calculated as the geometric mean of the test concentration with and without effect.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 748 mg/L
Additional information
A limit test was conducted to determine the acute toxicity of the Structural Analogue 01 to Daphnia magna according to EU Method C.2 (equivalent or similar to OECD Guideline 202), in compliance with GLP.
In thistest, 2 replicates of 10 Daphnia magna neonates were exposed to the test substance at a nominal concentration of 100 mg/L (mean measured concentration of 106.6 mg/L) under static conditions for 48 h.Immobility was recorded at 24 and 48 h.The analytical concentration of the test substance was measured by the TOC method at 0 and 48 h.
Under the test conditions, the 48 h EC50 and NOEC of the test substance were > 100 mg/L and >= 100 mg/L, respectively.
Reactive Black 5, a structural analogue to Reactive Blue 203, was tested for acute toxic effects on Daphnia magna according to OECD 202 at concentrations of 320, 560 and 1000 mg/L over an exposure time of 48 hours in a semi-static test under slightly acidic test conditions (pH 6) to avoid abiotic degradation of the test substance. No adverse effects were observed during this study at 320 and 560 mg/L. At 1000 mg/L all daphnids were immobile after 48 hours. The EC0 was 560 mg/L, the EC50 was calculated (as the geometric mean between 560 and 1000 mg/) to be 748 mg/L, the EC100 was 1000 mg/L.
Reactive Black 5-OH, the hydrolysis product of Reactive Black 5, a structural analogue to Reactive Blue 203, was tested for acute toxic effects on Daphnia magna according to OECD 202 at concentrations of 2 to 128 mg/L over an exposure time of 48 hours. No adverse effects were observed during this study. The EC0 and EC50 values are above the highest test concentration of 128 mg/L, tested.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
