3-phenyl-7-[4-(tetrahydrofurfuryloxy)phenyl]-1,5-dioxa-s-indacen-2,6-dione

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

None

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Principles of method if other than guideline:

None

GLP compliance:

yes

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):None

Analytical monitoring:

not specified

Details on sampling:

None

Vehicle:

yes

Details on test solutions:

Identity and concentration of auxiliary solvent for dispersal: Vorlösung in 1 % v/v Tween 80-Dimethylformamid

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM- Common name: Daphnia magna Straus.- Source: Laboratory culture originating from a strain supplied by the Institute National de recherche Chimique Appliquee (I.R.Ch.A.), France- Age at study initiation (mean and range, SD): young daphnids produced overnight were used.

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Post exposure observation period:

None

Hardness:

50 mg CaCO3/L

Test temperature:

no data

pH:

no data

Dissolved oxygen:

no data

Salinity:

not applicable

Nominal and measured concentrations:

% Concentration loss over test: 0.010, 0.10, 0.50 and 1.0 mg/l

Details on test conditions:

TEST SYSTEM- Test vessel: Glass jars- Type (delete if not applicable): open- Material, size, headspace, fill volume: 250 ml test solution- Aeration: None- Renewal rate of test solution (frequency/flow rate): None- No. of organisms per vessel: 0.5mg/l, four replicates- No. of vessels per concentration (replicates): 0.010, 0.10, 0.50 and 1.0 mg/l- Loading: 25 ml test solution per organismOTHER TEST CONDITIONS- Photoperiod: 16h light : 8h dark

Reference substance (positive control):

not specified

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 0.5 mg/L

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.5 mg/L

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 0.5 mg/L

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

>= 0.5 mg/L

Details on results:

None

Results with reference substance (positive control):

None

Reported statistics and error estimates:

None

Cumulative Immobilisation Data for Daphnia magna exposed for 48 hours to D-523:

Nominal Conc. Of test material (mg/l)	Cumulative immobilised Daphnia                                                (initial population : 10 per replicate)
	24 hours			48 hours
	No. per replicate	Total	%	No. per replicate	Total	%
Control                           R1	0	0	0	0	0	0
R2	0			0
Solvent Control          R1	0	0	0	0	0	0
R2	0			0
0.5                                    R1	0	0	0	0	0	0
R2	0			0
R3	0			0
R4	0			0

R1 - R4 = Replicates 1 -4

Validity criteria fulfilled:

not specified

Conclusions:

The NOEC and EC50 value after 48 hours were determined to be greater than 0.50 mg/l.

Executive summary:

FAT 40554 was tested for its acute toxicity to Daphnia magna in a 48 hours static test. The study was performed under Good Laboratory Practice (GLP) conditions in Switzerland.

The experiment was done according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adopted 4th April 1984, (issued 1993) and EU-Guideline 92/69, EEC Nr. L 383 A, C. 2, (29th Dec. 1992).

The nominal concentrations tested were0.010, 0.10, 0.50 and 1.0 mg/l test article, a vehicle and a blank control without any additions. 0.50mg/l was the highest test concentration that could be achieved due to the limited solubility of the test material in water and auxiliary solvent and having due regard to the amount of auxiliary solvent permitted in the test under the OECD Guidelines.

No immobilization and abnormal behaviour of the Daphnia was observed in the controls. The NOEC and EC0 value after 48 hours were determined to be 39 mg/l, the EC100 value are > 203 mg/l. The EC50 (48 hrs) value was found to be greater than 0.50 mg/l

According to Directive 93/21/EEC (adaptation to Directive 67/548/EEC) the ecotoxicological classification of FAT 40554 is not toxic to Daphnia magna after 48 hours.

Description of key information

The EC50 (48 hrs) value for FAT 4055411 in daphnia magna was found to be greater than 0.50 mg/l.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.5 mg/L

Additional information

In a key study, FAT 40554 was tested for its acute toxicity to Daphnia magna in a 48 hours static test. The study was performed under Good Laboratory Practice (GLP) conditions in Switzerland.

The experiment was done according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adopted 4th April 1984, (issued 1993) and EU-Guideline 92/69, EEC Nr. L 383 A, C. 2, (29th Dec. 1992).

The nominal concentrations tested were0.010, 0.10, 0.50 and 1.0 mg/l test article, a vehicle and a blank control without any additions. 0.50mg/l was the highest test concentration that could be achieved due to the limited solubility of the test material in water and auxiliary solvent and having due regard to the amount of auxiliary solvent permitted in the test under the OECD Guidelines.

No immobilization and abnormal behaviour of the Daphnia was observed in the controls. The NOEC and EC0 value after 48 hours were determined to be 39 mg/l, the EC100 value are > 203 mg/l. The EC50 (48 hrs) value was found to be greater than 0.50 mg/l

According to Directive 93/21/EEC (adaptation to Directive 67/548/EEC) the ecotoxicological classification of FAT 40554 is not toxic to Daphnia magna after 48 hours.