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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1988
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD study with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2-hydroxy-1-naphthaldehyde
EC Number:
211-902-0
EC Name:
2-hydroxy-1-naphthaldehyde
Cas Number:
708-06-5
Molecular formula:
C11H8O2
IUPAC Name:
2-hydroxynaphthalene-1-carbaldehyde
Details on test material:
Purity: 93.3 %

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
To improve distribution of the substance, 0.1 ml Tween 80/1 was used

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebrabärbling
- Strain: Hamilton-Buchanan
- Source: West Aquqrium, 3422 Bad Lauterberg
- Length at study initiation: 2.9 cm +- 0.21 cm
- Feeding during test: No


ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: yes
- Type and amount of food: Tetra Min, tetra Werke Melle
- Feeding frequency: twice a day ad libitum

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
21.3 - 23.0 °C
pH:
7.2 - 8.3
Dissolved oxygen:
5 - 9.4 mg/L
Nominal and measured concentrations:
nominal:
0, 0*, 0.1, 0.25, 0.35, 0.5, 0.7, 1.0, 10 mg/L
*solvent control with Tween 80/1
Details on test conditions:
TEST SYSTEM
- Test vessel: open glass vessel, 30cm x 22cm x 24cm, standing in a container with water with controlled temperature (22+-1°C), filled with 10 L water
- Aeration: no
- Type of flow-through : static
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates): 5 + 1 (with Tween 80/1)
- No. of vessels per vehicle control (replicates): 7

TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: according to ISO/DIS 7346/1

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 1 hours/day
- Light intensity: about 700 Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
2 - 4, 24, 48, 72 96 hours
checked for lethality, behaviour and appearance

Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
other: 0 % mortality
Effect conc.:
0 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
other: 0 % mortality
Effect conc.:
0.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 d
Dose descriptor:
other: 0 % mortality
Effect conc.:
0.25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
other: 0 % mortality
Effect conc.:
0.35 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
other: 10 % mortality
Effect conc.:
0.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
other: 100 % mortality
Effect conc.:
0.71 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
other: 100 % mortality
Effect conc.:
1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
other: 100 % mortality
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Results with reference substance (positive control):
not performed

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The LC50 for toxicity to fih is in the range from 0.5 to 0.71 mg/L
Executive summary:

The substance was tested for toxicity to fish according to OECD guideline 203 for a period of 96 hours. Additionally to the solvent controls, the following concentrations were used: 0.1, 0.25, 0.35, 0.5, 1.0 and 10 mg/L. Due to the bad solubility of the substance, Tween 80/1 was used to support the solubility. For each concentration 10 brachydanio rerio were used. In the concentration range from 0.5 to 10 mg/L lethality occured, whereas in the range from 0.1 to 0.35 mg/L only symptoms could be observed.

Based on the test results the following lethality-valued could be defined.

LC0 = 0.35 mg/L; LC50 = 0.5 - 0.71 mg/L; LC100 = 0.71 mg/L