2-[{6-[(4-amino-6-chloro-1,3,5-triazin-2-yl)(methyl)amino]-1-hydroxy-3-sulfonaphthalen-2-yl}diazenyl]naphthalene-1,5-disulfonic acid, lithium sodium salts

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From October 21, 2015 to June 02, 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge: An aeration tank at Joint Abbotsford Mission Environmental System (JAMES) wastewater treatment plant, Abbotsford, BC.
- Pretreatment: sludge was centrifuged, supernatant discarded, and sludge pellet re-suspended in mineral medium twice; sludge was brought up to the original volume; aerated in the dark at test temperature until use.

Duration of test (contact time):

28 d

Initial conc.:

60.5 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

- Test temperature: 16-22°C
- Suspended solids concentration: 0.03g/L

Reference substance:

benzoic acid, sodium salt

Remarks:

Sodium benzoate (CAS NO. 532-32-1)

Parameter:

% degradation (CO2 evolution)

Remarks on result:

other: Test Item cannot be considered readily biodegradable under the test conditions as the Test Item did not enter the 10 day window and as a result did not surpass 60% biodegradation by the end of the 10-day window.

Results with reference substance:

At Test completion(Day 28), the biodegradation was 68%.

At the end of the test, the difference between replicates was 6%. At Day 14 during the study, the biodegradation of the Reference Item was 63% and the percent biodegradation in the Toxicity Control was 45%. The mean oxygen uptake of the blanks was 14.6mg O2/L. The pH values were 7.1-7.6 between test initiation and completion.

Table 1. Percent Degradation of the Test and Reference Items Over the 28 Day Exposure Period

Treatment	Biodegradation at Start of 10-day Window	Biodegradation at End of 10-day Window	Biodegradation at Day 14*	Biodegradation at Test Completion
Test 1	N/A	N/A		-5%
Test 2	N/A	N/A		1%
Mean	N/A	N/A		-2%
Reference Item			63%	68%
Toxicity Control**			45%	46%

N/A-Not Applicable

*     Validity criteria for Reference Item and Toxicity Control are measured on Day14

**   Calculation of biodegradation in the Toxicity Control is based on the combined ThOD value for the Reference Item and Test Item

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

According to OECD 301F test method, CJ302 was not be considered readily biodegradable.

Executive summary:

This test using the procedures outlined in the Maxxam Study Plan for TOX1523 which is based on the SOP for the OECD 301F (BBY2 SOP-00023) and OECD 301F (OECD, 1992). The results of this OECD 301F test for CJ302 show that all validity criteria were met. CJ302 can not be considered readily biodegradable under the test conditions as CJ302 did not enter the 10 day window and a result did not surpass 60% biodegradation by the end of the 10-day window. Due to the stringency of this test, test items which do not achieve pass levels may still be biodegradable in the environment.

Description of key information

Not readily biodegradable. (OECD TG 301F).

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

At the end of the test, the difference between replicates was 6%. At Day 14 during the study, the biodegradation of the Reference Item was 63% and the percent biodegradation in the Toxicity Control was 45%. The mean oxygen uptake of the blanks was 14.6mg O2/L. The pH values were 7.1-7.6 between test initiation and completion.All test validity criteria were met.CJ302 can not be considered readily biodegradable under the test conditions as CJ302 did not enter the 10 day window and a result did not surpass 60% biodegradation by the end of the 10-day window. Due to the stringency of this test, test items which do not achieve pass levels may still be biodegradable in the environment.