Fats and Glyceridic oils, vegetable, winterized, reaction products with ammonia-ethanolamine reaction by-products

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

24-04-2013 to 23-05-2013

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: according to the guideline, under GLP

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

Principles of method if other than guideline:

The test substance was prepared by High Shear Mixing (7500 rpm for 15 minutes)

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: Severn Trent Water Plc sewage treatment plant Loughborough, Leicesterhire, UK
- Storage conditions: under aeration at 21 °C
- Storage length: <1 day
- Preparation of inoculum for exposure: washed twice by settlement and resuspended thereafter
- Pretreatment: none
- Concentration of sludge: suspended solids 3.1 g/L

Duration of test (contact time):

29 d

Details on study design:

TEST CONDITIONS
- Composition of medium: according to guideline
- Solubilising agent (type and concentration if used): none
- Test temperature: 21 °C
- pH: 7.2-7.6 (at day 0 after adjustment and day 28)
- pH adjusted: yes in all vessels
- Aeration of dilution water: yes
- Suspended solids concentration: 30 mg/L
- Continuous darkness: yes

TEST SYSTEM
- Number of culture flasks: 2 test substance +inoculum, 2 inoculum, 2 reference substance + inoculum, 1 toxicity control
- Method used to create aerobic conditions: bubbling of CO2-free air at 30-100 mL/min
- Details of trap for CO2 and volatile organics: 2 NaOH CO2-traps (Drechsel)

SAMPLING
- Sampling frequency:
CO2 on day 0, 2, 6, 8, 10, 14, 21, 28, 29 (second trap on day 0 and 29) analyses in triplicate
IC: on day 0 (stated to be not possible bacause of insolubility of the test item, but measurements included show accuracy within 5% of TC)
- Sterility check if applicable: not included
- Sample storage before analysis: none

CONTROL AND BLANK SYSTEM
- Inoculum blank: in duplicate
- Abiotic sterile control: not included
- Toxicity control: single vessel with test substance + reference substance + inoculum (20 mg C/L)

STATISTICS
student's t-test

Reference substance:

benzoic acid, sodium salt

Parameter:

% degradation (CO2 evolution)

Value:

0

Sampling time:

0 d

Parameter:

% degradation (CO2 evolution)

Value:

0

Sampling time:

29 d

Remarks on result:

other: after acidification of the medium

Details on results:

Test solutions all were pale brown dispersions. No undissolved test substance was visible.

Results with reference substance:

Degradation 62% on day 29

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The test substance attained 0 % degradation after 29 days and is therefore considered not readily biodegradable.

Executive summary:

The test substance was tested in a CO2 evolution test for 29 days. The test substance attained 0% degradation and is therefore considered not readily biodegradable.

Description of key information

The test substance was tested in a CO2 evolution test for 29 days. The test substance attained 0% degradation and is therefore considered not readily biodegradable (Harlan 2013).

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed