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EC number: 202-284-3 | CAS number: 93-89-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Basic toxicokinetics
Administrative data
- Endpoint:
- basic toxicokinetics
- Type of information:
- other: Expert statement
- Adequacy of study:
- key study
- Study period:
- 2015-06-09
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Expert statement, no study available
Data source
Reference
- Reference Type:
- other: Expert statement
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
- Principles of method if other than guideline:
- Expert statement
Test material
- Reference substance name:
- Ethyl benzoate
- EC Number:
- 202-284-3
- EC Name:
- Ethyl benzoate
- Cas Number:
- 93-89-0
- Molecular formula:
- C9H10O2
- IUPAC Name:
- ethyl benzoate
Constituent 1
Test animals
- Details on test animals or test system and environmental conditions:
- not applicable
Administration / exposure
- Details on exposure:
- not applicable
- Duration and frequency of treatment / exposure:
- not applicable
Doses / concentrations
- Remarks:
- Doses / Concentrations:
not applicable
- No. of animals per sex per dose / concentration:
- not applicable
- Positive control reference chemical:
- not applicable
- Details on study design:
- not applicable
- Details on dosing and sampling:
- not applicable
- Statistics:
- not applicable
Results and discussion
- Preliminary studies:
- not applicable
Toxicokinetic / pharmacokinetic studies
- Details on absorption:
- Generally, oral absorption is favoured for molecular weights below 500 g/mol. Furthermore, the relatively high water solubility of 720 mg/L enables the substance to readily dissolve in the gastrointestinal fluids, allowing direct uptake into the systemic circulation through aqueous pores or via carriage of the molecules across membranes with the bulk passage of water. The moderate log Pow value of ethyl benzoate is favourable for passive diffusion. Taken together, the physiochemical properties indicate that ethyl benzoate becomes bioavailable following the oral route. This assumption is confirmed by the results of the acute toxicity studies. These results did not lead to classification of the substance, but at least mortality was observed (leading to LD50 values of >2000 mg/kg bw).
Due to the low vapour pressure of ethyl benzoate it is unlikely that the substance will be available as a vapour, but if it is the case absorption via inhalation route is possible due to the water solubility and the moderate log Pow value, enabling uptake directly across the respiratory tract epithelium by passive diffusion.
Dermal absorption will also take place, favoured by the water solubility and the log Pow value, and also by the size of the molecule. Indeed, some clinical effects (diarrhoe in rabbits, salivation, tremor, muscular incoordination at a very high dose of 10000 mg/kg bw in cats) and mortality at doses over 2000 mg/kg bw in rabbits were reported (please refer to IUCLID section 7.2.3). - Details on distribution in tissues:
- The physicochemical properties of ethyl benzoate favour systemic absorption following oral, inhalative and dermal uptake.
After being absorbed into the body, ethyl benzoate is most likely distributed into the interior part of cells due to its slightly lipophilic properties (log Pow 2.59) and in turn the intracellular concentration may be higher than extracellular concentration particularly in adipose tissues.
Ethyl benzoate does not have an accumulative potential, as it is reported that benzoate esters are in general rapidly hydrolysed by carboxylesterases into benzoic acid and aliphatic alcohols. The enzyme mediated hydrolysis occurs in the blood, other body fluids and most body tissues, but predominantly carboxylesterases are found in hepatocytes (JECFA, 2002). As a chronic feeding study in rats conducted on benzoic acid did not reveal any signs of target organ toxicity or other indications for an accumulation in any organ or tissue, there is also no evidence for an accumulative property of this compound. Besides that, it is stated in literature that the breakdown products are further metabolized and excreted.
- Details on excretion:
- Ethyl benzoate will not be excreted in its unhydrolized form. The first degradation product, ethanol, and its metabolism and excretion are well known.
The second hydrolysis product, benzoic acid, is metabolised by conjugation to hippuric acid and renally excreted.
Metabolite characterisation studies
- Details on metabolites:
- As mentioned above, benzoate esters are generally hydrolysed by carboxylesterases into benzoic acid and aliphatic alcohols. This enzymatic reaction follows a first order kinetic. Ethyl benzoate was shown to have the longest half-life time of all alkyl benzoate esters. The experimentally determined half-life time for ethyl benzoate was 3.5 h (Nielsen & Bundgaard, 1987). Metabolism of ethyl benzoate is considered to be complete, and the metabolites are not supposed to be more toxic than the parent compound. This assumption is supported by the results obtained in the in vitro genetic toxicity tests conducted on ethyl benzoate and methyl benzoate in the presence of metabolic activation.
After hydrolysis of ethyl benzoate the resulting aliphatic alcohol (ethanol) is oxidized to a more polar metabolite (acetaldehyde) and further metabolized in the tricarboxylic acid cycle (JECFA, 2002). Benzoic acid is undergoing phase II reactions, and, after mainly being conjugated to hippuric acid, rapidly excreted via urine (IPCS & IOMC, 2000; Abdo et al., 1985).
Bioaccessibility (or Bioavailability)
- Bioaccessibility (or Bioavailability) testing results:
- Taken together, ethyl benzoates becomes bioavailable but will be metabolized and excreted completely and rapid.
Applicant's summary and conclusion
- Conclusions:
- Based on physicochemical characteristics, particularly water solubility and octanol-water partition coefficient, absorption by the dermal, oral and inhalation route is expected. This assumption is further supported by the results of the oral and dermal acute toxicity studies, revealing some effects at very high doses (above 2000 mg/kg bw). Bioaccumulation of ethyl benzoate or its breakdown products ethanol or benzoic acid will not occur. Enzymatic hydrolysis of ethyl benzoate is supposed to be complete. Ethanol is oxidized to acetaldehyde and degraded via the tricarboxylic acid cycle. Benzoic acid is conjugated to hippuric acid and excreted via urine.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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