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The acute toxicity of Hydrocarbons, C12-C16, n-alkanes, isoalkanes, alkenes to Rainbow trout (Oncorhynchus mykiss) was determined. The 96-h LL50 was > 100 mg/L loading rate WAF. In a reliable 48 -h acute toxicity immobilization study, Daphnia were exposed to Hydrocarbons, C12-C16, n-alkanes, isoalkanes, alkenes at measured WAF of 100 mg/L under static conditions following OECD 202 guidelines.  The 48 - h EL50was > 100 mg/L, based on mobility. The toxicity of Hydrocarbons, C12-C16, n-alkanes, isoalkanes, alkenes to Algae, Pseudokirchneriella subcapitata, was determined. The 72-h EL50, based on growth was > 100 mg/L and the 72-h NOEC, based on growth was 100 mg/L WAF.