Tetrasodium 7-[[2-[(aminocarbonyl)amino]-4-[[4-chloro-6-[[3-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]phenyl]azo]naphthalene-1,3,6-trisulphonate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

From June 29 to August 24, 1995

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

The test was conducted by means of Read Across approach. The reliability of the source study report is 2. Further information was attached at section 13

Qualifier:

according to guideline

Guideline:

other: ISO 11734

GLP compliance:

not specified

Oxygen conditions:

anaerobic

Inoculum or test system:

anaerobic bacteria

Details on inoculum:

- Source of inoculum: Municipal sewage Kriftel
- Storage conditions: at 35°C +/- 2°C thermostat Incubator, the temperature has remained during the test in range of 33.0°C- 37.0°C
- Storage length: 7 days- Preparation of inoculum for exposure: bacterial biomass anaerobic digester of a semi-continuous operation, through a 0.5 mm sieve to separate coarse particles, washed, resuspended to the DOC value of the class does not exceed 10 mg/L (C)100 mL/L of anaerobic inoculum suspension with a solids content of 32.4 g/L, corresponding to 3.24 The final volume in the dry mass of the audit method.
- Pretreatment:the inoculum was 7 days for depletion of carbon in the incubatormixed at 35 ± 2 °C- Type and size of filter used, if any: 0.5 mm

Duration of test (contact time):

ca. 56 d

Initial conc.:

113 mg/L

Based on:

DOC

Initial conc.:

500 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

CO2 evolution

Parameter followed for biodegradation estimation:

CH4 evolution

Details on study design:

TEST CONDITIONS
-Test vessel: 1000 mL glass tank with magnetic stirrer
- Composition of medium: ISO 11734 Punkt 6.2 Testmedium
- Test temperature: 33 - 37 °C
- pH: 7.2
- pH adjusted: yes
- Suspended solids concentration: 100 mL/L
- Stock solution: 2000 mg/L; 2000 mg/800 mL weighed in highly purified water, measured pH 4.3; adjusted to pH 7.2, stirred for 2 hours at 22°C, measured pH 7.2, filled up to 1000 mL and used as stock solution.

TEST SYSTEM
- Number of culture flasks/concentration: 3
- Details of trap for CO2 and volatile organics if used: graduated gas mouse
- Other:BLANK SYSTEM
- Inoculum blank: yes

Reference substance:

benzoic acid, sodium salt

Parameter:

% degradation (CO2 evolution)

Value:

< 20

Sampling time:

56 d

Parameter:

% degradation (CH4 evolution)

Value:

< 20

Sampling time:

56 d

Results with reference substance:

> 60 % gas formation

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

A significant anaerobic biodegradation was not observed. The formation of gas within 56 days remained below 20% of the theoretical maximum.

Executive summary:

Method

The test substance was tested for biodegradation in anaerobic conditions according to the ISO 11734.

Results

The formation of gas within 56 days remained below 20 % of the theoretical maximum. A significant anaerobic biodegradation was not observed.

Description of key information

Not readily biodegradable