Tetrasodium 7-[[2-[(aminocarbonyl)amino]-4-[[4-chloro-6-[[3-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]phenyl]azo]naphthalene-1,3,6-trisulphonate

Partition coefficient

Link to relevant study record(s)

Reference

Endpoint:

partition coefficient

Type of information:

experimental study

Adequacy of study:

key study

Study period:

The 7th of June, 2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 107 (Partition Coefficient (n-octanol / water), Shake Flask Method)

Version / remarks:

adopted 27.07.95

GLP compliance:

yes

Type of method:

flask method

Partition coefficient type:

octanol-water

Analytical method:

photometric method

Type:

Pow

Partition coefficient:

ca. 0.013

Temp.:

20 °C

pH:

6.05

Type:

log Pow

Partition coefficient:

ca. -2.053

Temp.:

20 °C

pH:

6.05

Details on results:

A first scan of the solutions was performed in the range 190 – 800 nm to choose the best wavelength peak for the quantitative measurements. In the case of the test item: lambda= 416 nm.Concentration of the n-octanol phase was very low: even applying direct measurement of the n-octanol phase, absorbance values were far below the lower limit of the spectrometrer's sensibility.

Conclusions:

log Pow = - 2.0528

Executive summary:

Method

Partition coefficient was determined by OECD Guideline 107 (Partition Coefficient (n-octanol / water), Shake Flask Method), adopted 27.05.95.

n- octanol and double distilled water were pre saturated: enough quantity of double distilled water was mixed with n-octanol at room temperature and kept under agitation during 24 hours, then allowing phase separation. Analogous procedure was followed for n-octanol with addition of double distilled water.

Approximately exactly 0.1 g test sample was weighed and dissolved with double distilled water saturated in n-octanol to 250 ml in a volumetric flask (mother solution). pH was recorded.

Results

The average Pow measured is 0.012691 at pH of 6.05, the average log Pow measured is - 2.0528 at 20 °C.

Description of key information

logKow = - 2.0528 

Key value for chemical safety assessment

Log Kow (Log Pow):

-2.053

at the temperature of:

20 °C

Additional information

Partition coefficient was determined by OECD Guideline 107 (Partition Coefficient (n-octanol / water), Shake Flask Method), adopted 27.05.95.

n- octanol and double distilled water were pre saturated: enough quantity of double distilled water was mixed with n-octanol at room temperature and kept under agitation during 24 hours, then allowing phase separation. Analogous procedure was followed for n-octanol with addition of double distilled water.