Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
16 March 1994 to 11 April 1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
yes
Remarks:
Preparation of the inoculum: higher sludge weight after the washing procedure (Sludge concentrations in the test according to the guidelines) * 4 instead of 5 test concentrations
GLP compliance:
yes
Specific details on test material used for the study:
Code number: FAT - 20035/C
EN-No.: 140200.31
Appearance: solid
Solubility: 20g/l [in water]
Storage: room temperature
Expiration date: 10/98
Analytical monitoring:
not specified
Vehicle:
no
Test organisms (species):
activated sludge
Details on inoculum:
Pretreatment: 3 times washing with tap water and addition of synthetic sewage (a day before the main test)
Test type:
static
Water media type:
not specified
Limit test:
yes
Total exposure duration:
3 h
Post exposure observation period:
None
Hardness:
Not available
Test temperature:
22.0 °C
pH:
7.8 - 7.9
Dissolved oxygen:
Not available
Salinity:
Not available
Nominal and measured concentrations:
Test concentrations :
320, 100, 32 and 10 mg/l of the test substance
32, 10 and 3.2 mg/l of the reference substance
Details on test conditions:
TEST SYSTEM
- Aeration:0.1 - 0.2 l/min by pasteur-pipette

Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol (Merck 821768)
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
280 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Details on results:
The IC20 (3 h) of the test substance FAT - 20035/C: 50.0 mg/L
Results with reference substance (positive control):
The IC50 (3 h) of the reference substance 3,5-Dichlorophenol: 11.0 mg/l
Reported statistics and error estimates:
None
Validity criteria fulfilled:
yes
Conclusions:
IC50 (3 h) of FAT - 20035/C is 280.0 mg/L.

Executive summary:

The toxicity of FAT 20035/C to activated sludge was determined in a 3 hours respiration inhibition test according to the EEC Method No. L 133/118 -122 and to the OECD Guideline No. 209. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations. The test substance concentrations tested were 320, 100, 32 and 10 mg/l. Based on the study results, IC20 (3 h) and IC50 (3 h) of FAT - 20035/C is 50 and 280.0 mg/L, respectively, while that of the reference substance 3,5-Dichlorophenol is 11.0 mg/l.

Description of key information

The IC 50 value for test substance was found to be 181 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
181 mg/L

Additional information

A GLP-compliant study was conducted to determine the toxicity of FAT 20035/D to activated sludge in a 3 hours respiration inhibition test according to the OECD Guideline No. 209, and to the EEC Method No. L 87/302. The test was performed in compliance with the Good Laboratory Practice (GLP). The test substance concentrations tested were 320,100, 32 and 10 mg/l. The reference substance, 3,5-Dichlorophenol was tested in concentrations of 32, 10 and 3.2 mg/l. Based on the study results, the IC20 (3 h) and IC50 (3 h) of FAT 20035/D 30 and 181 mg/l, respectively, while that of reference substance 3,5-Dichlorophenol is 14 mg/l.


Another GLP study was conducted on FAT 20035/C to determined IC50 value in a 3-hours respiration inhibition test according to the EEC Directive L 133/118 and OECD guideline 209. The test substance concentrations tested were 320, 100, 32 and 10 mg/l. The results showed an IC50 (3 h) of the test substance FAT 20035/D as 280 mg/l.


In several other supporting studies, BST values greater than 300 mg/L were found.