[1-[[(2-hydroxyphenyl)imino]methyl]-2-naphtholato(2-)-N,O,O']copper

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1978

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

no

GLP compliance:

no

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

[1-[[(2-hydroxyphenyl)imino]methyl]-2-naphtholato(2-)-N,O,O']copper

EC Number:

239-763-1

EC Name:

[1-[[(2-hydroxyphenyl)imino]methyl]-2-naphtholato(2-)-N,O,O']copper

Cas Number:

15680-42-9

Molecular formula:

C17H11CuNO2

IUPAC Name:

[1-[[(2-hydroxyphenyl)imino]methyl]-2-naphtholato(2-)-N,O,O']copper

Details on test material:

Batch: EN 75024
physical state: solid

Specific details on test material used for the study:

Batch: EN 75024
physical state: solid

Test animals

Species:

rat

Strain:

other: Tif:RAIf

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: raised on the premises of the testing facility
- Age at study initiation: 7 to 8 weeks
- Weight at study initiation: ca 180 g for males and ca 170g for females
- Fasting period before study: overnight
- Housing: groups of five
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 4 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 + 1°C
- Humidity (%): 55 + 5 %
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): 14/10

IN-LIFE DATES: From: 1978-10-17 To: 1978-11-8

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

polyethylene glycol

Details on oral exposure:

MAXIMUM DOSE VOLUME APPLIED: 10 or 20 ml/kg

DOSAGE PREPARATION (if unusual): Before treatment the suspension was homogeneously dispersed with an Ultra-Turrax and during treatment it was kept stable with a magnetic stirrer.

Doses:

3000, 4000 and 5000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Weighing daily, observations on hour 1,2,4, 6 and 24, then once daily until day 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs (Sedation, Dyspnoea, Dacryorrhoea, Chromodacryorrhoea, Rinorrhoea, Epistaxis, Exophthalmos,Salivation, Ruffled fur, Pallor, Cyanosis, Diarrhoea, Body position (ventral/lateral/curved), Ataxia, Trismus, Tremor, Tonic clonic muscle spasms Convulsion), body weight

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: No mortality occurred.

Mortality:

No mortality occurred.

Clinical signs:

other: Effects occurred at all dose levels in a dose dependent manner. The animals recovered within 8 days. Sedation: up to 24h Dyspnoea: up to up to 8 days Exophthalmos: up to 6 days Ruffled fur: up to 8 days Curved body position: up to 8 days

Gross pathology:

No substance related gross organ changes were seen.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met