5-({4-chloro-6-[ethyl(phenyl)amino]-1,3,5-triazin-2-yl}amino)-4-hydroxy-3-[(1-sulfo-2-naphthyl)diazenyl]naphthalene-2,7-disulfonic acid, lithium sodium salts

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From October 29, 2019 to January 02, 2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Source: AZOO BIO CORPORATION
- Length at study initiation: 1.0-2.0 cm
- Acclimation period: 10 days

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Nominal and measured concentrations:

Nominal concentrations for the range-finding test are 0.16, 0.80, 4.00, 20.00 and 100.00 mg/L.
Nominal concentration for the limit test is 100.00 mg/L.

Details on test conditions:

- Test volume: 500 mL
- Photoperiod: 16-hours : 8-hours (Light : Dark)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Table 1. The number of fish died at each observation time for the range-finding test

Group	Nominal conc. (mg/L)	Number of death (individual)
		0 hour	2 hours	5 hours	24 hours	30 hours	48 hours	54 hours	72 hours	78 hours	96 hours
Control group	0.00	0	0	0	0	0	0	0	0	0	1
Test group	0.16	0	0	0	0	0	0	0	0	0	1
	0.80	0	0	0	0	0	0	0	0	0	1
	4.00	0	0	0	0	0	0	0	0	0	1
	20.00	0	0	0	0	1	1	1	1	1	1
	100.00	0	0	0	0	0	0	0	1	1	1

Table 2. The percentages mortality of fish at each observation time for the range-finding test

Group	Nominal conc. (mg/L)	Mortality (%)
		0 hour	2 hours	5 hours	24 hours	30 hours	48 hours	54 hours	72 hours	78 hours	96 hours
Control group	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	14.29
Test group	0.16	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	14.29
	0.80	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	14.29
	4.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	14.29
	20.00	0.00	0.00	0.00	0.00	14.29	14.29	14.29	14.29	14.29	14.29
	100.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	14.29	14.29	14.29

Table 3. Light intensity, temperature, dissolved oxygen and pH value for the range-finding test

Group	Nominal conc. (mg/L)	Time (hour)	Light intensity (lux)	Temperature (°C)	Dissolved oxygen level (%)	pH
Control group	0.00	0	900	25.0	99.0	7.81
		24	890	24.9	87.1	7.61
		48	910	25.0	86.0	7.28
		72	880	24.9	72.9	7.15
		96	890	24.9	74.3	7.17
Test group	100.00	0	900	25.0	99.3	7.80
		24	890	24.9	85.0	7.53
		48	910	25.0	88.1	7.35
		72	880	24.9	74.5	7.10
		96	890	24.9	76.1	7.18

Table 4. The number of fish died at each observation time for the limit test

Group	Nominal conc. (mg/L)	Number of death (individual)
		0 hour	2 hours	5 hours	24 hours	30 hours	48 hours	54 hours	72 hours	78 hours	96 hours
Control group	0.00	0	0	0	0	0	0	0	0	0	0
Test group	100.00	0	0	0	0	0	0	0	0	0	0

Table 5. The percentages mortality of fish at each observation time for the limit test

Group	Nominal conc. (mg/L)	Mortality (%)
		0 hour	2 hours	5 hours	24 hours	30 hours	48 hours	54 hours	72 hours	78 hours	96 hours
Control group	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
Test group	100.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00

Table 6. Light intensity, temperature, dissolved oxygen and pH value for the limit test

Group	Nominal conc. (mg/L)	Time (hour)	Light intensity (lux)	Temperature (°C)	Dissolved oxygen level (%)	pH
Control group	0.00	0	880	25.0	99.0	7.11
		24	890	25.0	94.5	7.16
		48	860	25.0	90.2	7.10
		72	880	25.0	88.0	7.06
		96	900	25.0	86.8	7.11
Test group	100.00	0	880	25.0	99.7	7.15
		24	890	25.0	93.2	7.18
		48	860	25.0	90.8	7.13
		72	880	25.0	87.1	7.09
		96	900	25.0	88.9	7.15

Validity criteria fulfilled:

yes

Conclusions:

According to OECD 203 test method, the NOEC of CJ306 was 100 mg/L.

Executive summary:

This test using the procedures outlined in the SuperLab Study Plan for M62-190900035001EN which is based on the OECD 203 (OECD, 2019).All test validity criteria were met. At the range-finding test, the mortality of 0.16-100.00 mg/L were all 14.29% and 96-hr LC50 was greater than 100.00 mg/L. Base on this result of range-finding test, the highest concentration of range-finding test (100.00 mg/L) was used to conduct the limit test. Therefore, the limit test was conducted and results showed that the mortality of 100.00 mg/L was 0.00% at the end of test. Under the conditions of this test, the NOEC of CJ306 was 100 mg/L.

Description of key information

The NOEC of CJ306 was 100 mg/L for Danio rerio (OECD TG203).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

The mortalities of control group were smaller than 10% in both range-finding and limit test. The temperature deviation were within ± 2 °C and dissolved oxygen were ≥ 60 %. All test validity criteria were met. At the range-finding test, the mortality of 0.16-100.00 mg/L were all 14.29% and 96-hr LC50 was greater than 100.00 mg/L. Base on this result of range-finding test, the highest concentration of range-finding test (100.00 mg/L) was used to conduct the limit test. Therefore, the limit test was conducted and results showed that the mortality of 100.00 mg/L was 0.00% at the end of test. Under the conditions of this test, the NOEC of CJ306 was 100 mg/L.