Gon-4-ene-3,17-dione, 13-ethyl-

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

(+)-13-Ethylgon-4-ene-3,17-dione

EC Number:

606-833-1

Cas Number:

21800-83-9

Molecular formula:

C19H26O2

IUPAC Name:

(+)-13-Ethylgon-4-ene-3,17-dione

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

Test item: Levodione
CAS number: 21800-83-9
Physical state: Solid, crystalline powder
Colour: White
Storage: At a temperature of 15 to 30 °C

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

Test species: Pseudokirchneriella subcapitata (Korshikov) F.Hindák (formerly known as Selenastrum capricornutum)
Source: UTEX The Culture Collcetion of Algae (USA)
Batch: UTEX ID. number: 1648
Date of arriving: 28 November 2014

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

The temperature data were harvested by Extech SD200 datalogger.

pH:

At the beginning of the test the pH values were measured in the remaining test solution of the concurrent test vessels at each dilution level. At the end of the test the pH values were measured in each test vessel. The pH of test solutions was not adjusted at the beginning of the test.

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

8.42 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC20

Effect conc.:

4.88 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

3.67 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 4

Duration:

72 h

Dose descriptor:

LOEC

Remarks on result:

not determinable

Effect concentrations 5

Duration:

72 h

Dose descriptor:

NOEC

Remarks on result:

not determinable

Details on results:

The No Observed Effect Concentration and Lowest Observed Effect Concentration for the test item were calculated by ToxRat® software with Fisher’s exact binominal test with Bonferroni correction for specific growth rate and yield. The results were not conclusive or not determinable.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

According to the Council Regulation (EC) 440/2008 Levodione is hazardous to the aquatic environment and classified into the second acute toxic hazardous category [GHS Acute aquatic hazard Category Acute II. – 72 h EC50 (for algae or other aquatic plants) > 1 mg/L but ≤ 10 mg/L] [7, 8].