[No public or meaningful name is available]

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): Gelb LD 6259
- Lot/batch No.: Lot 20980
- Purity test date: 87.6 - 89.1 area %

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 239.94 mg test substance were dissolved in 2 l test medium and stirred for 10 minutes at 20 +/- 2 °C

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquée, France and cultured since 1978 at the test facility
- Age at study initiation (mean and range, SD): < 24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

2.2 - 3.2 mmol/l

Test temperature:

19.6 - 20.5 °C

pH:

7.9 - 8.1

Dissolved oxygen:

8.6 - 8.8 mg/l

Nominal and measured concentrations:

Nominal test concentration: 120 mg/l
The analytically determined concentration was within 20 % of the nominal concentration.
Analytically determined concentrations:
0 hours: 119.7 mg/l
48 hours: 124.5 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers (250 ml)
- Fill volume: 200 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.025 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic fresh water (M 4 medium )
- Ca/mg ratio: 4:1
- Conductivity: 550 - 650 µS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: 20 - 850 lux at a wave length of 400 - 750 nm


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 24 and 48 hours

TEST CONCENTRATIONS
- Results used to determine the conditions for the definitive study: EC50 (48 h) > 100 mg/l

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

The EC50(48h) of the reference substance potassium dichromate was 1.13 mg/L.
(Date of the last control experiment: 12 Feb 2009, project-no. 50E0063/043039)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In conclusion the 48h EC50 for Gelb LD 6259 with Daphnia magna were >120 mg/L based on the nominal concentration of the test substance and >122 mg/L based on the mean of analytically determined concentrations.