[No public or meaningful name is available]

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes (incl. QA statement)

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): Gelb LD 6259
- Lot/batch No.: 0817VP01
- Analytical purity: The IR spectrum shows the expected bands; the 1H and 13C NMR spectra show expected signals for the current structure (BASF report No. 08L00346
- Physical state: solid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): municipal activated sludge taken from the waste water treatment plant of Mannheim (Baden-Wuertemberg, Germany).
- Preparation of inoculum for exposure: the inoculum was sieven (mesh size 1 mm) and pre-aerated
- Concentration of sludge: 30 mg/l

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

86 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

20 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Test temperature: 22 +/- 2 °C


TEST SYSTEM
- Number of culture flasks/concentration: 2


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

< 10

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: poorly biodegradable

Conclusions:

The test substance was poorly biodegradable and according to OECD criteria "not readily biodegradable " in this test.