Tetrasodium 4-amino-5-hydroxy-6-[[2-methoxy-5-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]azo]-3-[[4-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2,7-disulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

January 2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to other study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method of administration: Direct weighing
- Water for dilution: Synthetic fresh water in accordance with ISO.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Strain: HAMILTON BUCHANAN
- Source: Bio International B.V. (Netherlands)
- Age at study initiation (mean and range, SD): ~ 4 mo
- Length at study initiation (length definition, mean, range and SD): 2.5-3.5 cm

ACCLIMATION
- Acclimation conditions (same as test or not): Yes
- Keeping water: Synthetic fresh water in accordance with ISO
- Mortality: <2 % while kept

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

No

Hardness:

14.1 °dH

Test temperature:

20.8-22 °C

pH:

7.1-8

Dissolved oxygen:

7.5-8.8 mg/L
Oxygen [% saturation]=86.5-101.3

Salinity:

No data

Nominal and measured concentrations:

Nominal: 1000 and 3160 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Test vessels (300 x 135 x 200 mm)
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: 5 L test medium
- Aeration: Yes
- Type of flow-through (e.g. peristaltic or proportional diluter): Static
- Method of administration: Direct weighing


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic fresh water in accordance with ISO

OTHER TEST CONDITIONS
- Photoperiod: 16 h light/8 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality, visible effects on appearance and behaviour

TEST CONCENTRATIONS
- Test concentrations: 1000, 3160 mg/L

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: -

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1 777.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: geometric mean of LC0 and LC100

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

3 160 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: -

Details on results:

- Behavioural abnormalities: No effects at 1000 mg/L, at 3160 not observable due to staining of test medium

Validity criteria fulfilled:

yes

Conclusions:

Under the test conditions, the 96 h LC0 value of the test substance (including inorganic components) was determined to be 1000 mg/L.

Executive summary:

A study was performed to assess the acute toxicity of test substance to Zebra fish (Brachydanio rerio) under static conditions. The study was conducted in accordance with EU Method C.1. in compliance with GLP.

 

Groups of ten fish of the recommended size were exposed to limit test concentration of nominally 1000 and 3160 mg/L of test substance dissolved in synthetic fresh water.

Observations were made on the number of dead fish and for the visible effects on appearance and behaviour of fish after 0, 24, 48, 72 and 96 h of exposure. 

 

Under the test conditions, the 96 h LC0, LC 50 and LC100 values of the test substance were determined to be 1000, 1777.6, and 3160 mg/L, respectively.

Description of key information

The 96 h LC0, LC 50 and LC100 values of the test substance were determined to be1000, 1777.6, and 3160 mg/L, respectively.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

1 777.6 mg/L

Additional information

A study was performed to assess the acute toxicity of test substance to Zebra fish (Brachydanio rerio) under static conditions. The study was conducted in accordance with EU Method C.1. in compliance with GLP.

 

Groups of ten fish of the recommended size were exposed to limit test concentration of nominally 1000 and 3160 mg/L of test substance dissolved in synthetic fresh water.

Observations were made on the number of dead fish and for the visible effects on appearance and behaviour of fish after 0, 24, 48, 72 and 96 h of exposure. 

 

Under the test conditions, the 96 h LC0, LC 50 and LC100 values of the test substance were determined to be1000, 1777.6, and 3160 mg/L, respectively.